Literature DB >> 23023743

Probing the role of sigma π interaction and energetics in the catalytic efficiency of endo-1,4-β-xylanase.

Raushan Kumar Singh1, Manish Kumar Tiwari, In-Won Kim, Zhilei Chen, Jung-Kul Lee.   

Abstract

Chaetomium globosum endo-1,4-β-xylanase (XylCg) is distinguished from other xylanases by its high turnover rate (1,860 s(-1)), the highest ever reported for fungal xylanases. One conserved amino acid, W48, in the substrate binding pocket of wild-type XylCg was identified as an important residue affecting XylCg's catalytic efficiency.

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Year:  2012        PMID： 23023743      PMCID： PMC3502898          DOI： 10.1128/AEM.02261-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  The endoxylanases from family 11: computer analysis of protein sequences reveals important structural and phylogenetic relationships.

Authors:  Amalia Sapag; Johan Wouters; Christophe Lambert; Pablo de Ioannes; Jaime Eyzaguirre; Eric Depiereux
Journal:  J Biotechnol       Date:  2002-05-09       Impact factor: 3.307

Review 2.  A new look at xylanases: an overview of purification strategies.

Authors:  Paula Sá-Pereira; Helena Paveia; Maria Costa-Ferreira; Maria Aires-Barros
Journal:  Mol Biotechnol       Date:  2003-07       Impact factor: 2.695

3.  Mapping glycoside hydrolase substrate subsites by isothermal titration calorimetry.

Authors:  Gennady Zolotnitsky; Uri Cogan; Noam Adir; Vered Solomon; Gil Shoham; Yuval Shoham
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-26       Impact factor: 11.205

4.  Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement.

Authors:  Nobuhiko Watanabe; Toshihiko Akiba; Ryuta Kanai; Kazuaki Harata
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-06-20

5.  Crystallographic analysis shows substrate binding at the -3 to +1 active-site subsites and at the surface of glycoside hydrolase family 11 endo-1,4-beta-xylanases.

Authors:  Elien Vandermarliere; Tine M Bourgois; Sigrid Rombouts; Steven Van Campenhout; Guido Volckaert; Sergei V Strelkov; Jan A Delcour; Anja Rabijns; Christophe M Courtin
Journal:  Biochem J       Date:  2008-02-15       Impact factor: 3.857

6.  Understanding the structural basis for substrate and inhibitor recognition in eukaryotic GH11 xylanases.

Authors:  Maria Vardakou; Claire Dumon; James W Murray; Paul Christakopoulos; David P Weiner; Nathalie Juge; Richard J Lewis; Harry J Gilbert; James E Flint
Journal:  J Mol Biol       Date:  2007-11-12       Impact factor: 5.469

7.  Mutagenesis and subsite mapping underpin the importance for substrate specificity of the aglycon subsites of glycoside hydrolase family 11 xylanases.

Authors:  Annick Pollet; Stijn Lagaert; Elena Eneyskaya; Anna Kulminskaya; Jan A Delcour; Christophe M Courtin
Journal:  Biochim Biophys Acta       Date:  2010-01-21

8.  Structural comparison of two major endo-1,4-xylanases from Trichoderma reesei.

Authors:  A Törrönen; J Rouvinen
Journal:  Biochemistry       Date:  1995-01-24       Impact factor: 3.162

9.  Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase.

Authors:  W W Wakarchuk; R L Campbell; W L Sung; J Davoodi; M Yaguchi
Journal:  Protein Sci       Date:  1994-03       Impact factor: 6.725

10.  Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry.

Authors:  S Miao; L Ziser; R Aebersold; S G Withers
Journal:  Biochemistry       Date:  1994-06-14       Impact factor: 3.162
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  2 in total

1.  Probing the role of cation-π interaction in the thermotolerance and catalytic performance of endo-polygalacturonases.

Authors:  Tao Tu; Yeqing Li; Xiaoyun Su; Kun Meng; Rui Ma; Yuan Wang; Bin Yao; Zhemin Lin; Huiying Luo
Journal:  Sci Rep       Date:  2016-12-08       Impact factor: 4.379

2.  Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis.

Authors:  Shuai You; Chun-Chi Chen; Tao Tu; Xiaoyu Wang; Rui Ma; Hui-Yi Cai; Rey-Ting Guo; Hui-Ying Luo; Bin Yao
Journal:  Biotechnol Biofuels       Date:  2018-06-08       Impact factor: 6.040
  2 in total

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