Literature DB >> 23022558

Equilibrium self-association of tropomyosin.

Walter F Stafford1, Eunhee Lee, Philip Graceffa.   

Abstract

It has recently been reported that tropomyosin exists exclusively as a dimer in physiological salt conditions. It is shown in the present work using analytical ultracentrifugation that, on the contrary, tropomyosin is in equilibrium between monomer, dimer and tetramer with a weak tendency to dimerize and tetramerize. Such a finding has consequences for the assembly of the tropomyosin-actin complex.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year:  2012        PMID: 23022558      PMCID: PMC3500083          DOI: 10.1016/j.febslet.2012.08.035

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  26 in total

Review 1.  Cooperativity in the Ca2+ regulation of muscle contraction.

Authors:  Michael A Geeves; Sherwin S Lehrer
Journal:  Results Probl Cell Differ       Date:  2002

2.  Analysis of heterologous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium and kinetic constants.

Authors:  Walter F Stafford; Peter J Sherwood
Journal:  Biophys Chem       Date:  2004-03-01       Impact factor: 2.352

3.  Tropomyosin is a tetramer under physiological salt conditions.

Authors:  Ingrid Lassing; Louise Hillberg; Anna-Stina Höglund; Roger Karlsson; Clarence Schutt; Uno Lindberg
Journal:  Cytoskeleton (Hoboken)       Date:  2010-09

4.  The content of troponin, tropomyosin, actin, and myosin in rabbit skeletal muscle myofibrils.

Authors:  J D Potter
Journal:  Arch Biochem Biophys       Date:  1974-06       Impact factor: 4.013

5.  Reconstitution of troponin activity from three protein components.

Authors:  M L Greaser; J Gergely
Journal:  J Biol Chem       Date:  1971-07-10       Impact factor: 5.157

6.  Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

Authors:  L M Siegel; K J Monty
Journal:  Biochim Biophys Acta       Date:  1966-02-07

7.  Physicochemical studies on the aggregation of bovine cardiac tropomyosin with ionic strength.

Authors:  W D McCubbin; C M Kay
Journal:  Can J Biochem       Date:  1969-04

8.  Tropomyosin: crystal structure, polymorphism and molecular interactions.

Authors:  D L Caspar; C Cohen; W Longley
Journal:  J Mol Biol       Date:  1969-04-14       Impact factor: 5.469

9.  Equilibrium of the actin-tropomyosin interaction.

Authors:  A Wegner
Journal:  J Mol Biol       Date:  1979-07-15       Impact factor: 5.469

10.  Effects of an interchain disulfide bond on tropomyosin structure: intrinsic fluorescence and circular dichroism studies.

Authors:  S S Lehrer
Journal:  J Mol Biol       Date:  1978-01-15       Impact factor: 5.469

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