BACKGROUND: The neuronal circuitry underlying sleep is poorly understood. Although dopamine (DA) is thought to play a key role in sleep/wake regulation, the identities of the individual DA neurons and their downstream targets required for this process are unknown. RESULTS: Here, we identify a DA neuron in each PPL1 cluster that promotes wakefulness in Drosophila. Imaging data suggest that the activity of these neurons is increased during wakefulness, consistent with a role in promoting arousal. Strikingly, these neurons project to the dorsal fan-shaped body, which has previously been shown to promote sleep. The reduced sleep caused by activation of DA neurons can be blocked by loss of DopR, and restoration of DopR expression in the fan-shaped body can rescue the wake-promoting effects of DA in a DopR mutant background. CONCLUSIONS: These experiments define a novel arousal circuit at the single-cell level. Because the dorsal fan-shaped body promotes sleep, these data provide a key link between wake and sleep circuits. Furthermore, these findings suggest that inhibition of sleep centers via monoaminergic signaling is an evolutionarily conserved mechanism to promote arousal.
BACKGROUND: The neuronal circuitry underlying sleep is poorly understood. Although dopamine (DA) is thought to play a key role in sleep/wake regulation, the identities of the individual DA neurons and their downstream targets required for this process are unknown. RESULTS: Here, we identify a DA neuron in each PPL1 cluster that promotes wakefulness in Drosophila. Imaging data suggest that the activity of these neurons is increased during wakefulness, consistent with a role in promoting arousal. Strikingly, these neurons project to the dorsal fan-shaped body, which has previously been shown to promote sleep. The reduced sleep caused by activation of DA neurons can be blocked by loss of DopR, and restoration of DopR expression in the fan-shaped body can rescue the wake-promoting effects of DA in a DopR mutant background. CONCLUSIONS: These experiments define a novel arousal circuit at the single-cell level. Because the dorsal fan-shaped body promotes sleep, these data provide a key link between wake and sleep circuits. Furthermore, these findings suggest that inhibition of sleep centers via monoaminergic signaling is an evolutionarily conserved mechanism to promote arousal.
Authors: Hidehiko K Inagaki; Shlomo Ben-Tabou de-Leon; Allan M Wong; Smitha Jagadish; Hiroshi Ishimoto; Gilad Barnea; Toshihiro Kitamoto; Richard Axel; David J Anderson Journal: Cell Date: 2012-02-03 Impact factor: 41.582
Authors: Shannon H Cole; Ginger E Carney; Colleen A McClung; Stacey S Willard; Barbara J Taylor; Jay Hirsh Journal: J Biol Chem Date: 2005-02-03 Impact factor: 5.157
Authors: Michael J Krashes; Shamik DasGupta; Andrew Vreede; Benjamin White; J Douglas Armstrong; Scott Waddell Journal: Cell Date: 2009-10-16 Impact factor: 41.582
Authors: Anna Phan; Connon I Thomas; Molee Chakraborty; Jacob A Berry; Naomi Kamasawa; Ronald L Davis Journal: Neuron Date: 2018-11-28 Impact factor: 17.173
Authors: Yuhua Shang; Nathan C Donelson; Christopher G Vecsey; Fang Guo; Michael Rosbash; Leslie C Griffith Journal: Neuron Date: 2013-10-02 Impact factor: 17.173