| Literature DB >> 2302170 |
V E Piskarev1, A M Shuster, A G Gabibov, A G Rabinkov.
Abstract
A method for the rapid preparative isolation of highly purified avidin using h.p.l.c. on a powerful cation-exchanger TSK SP-5PW column has been developed. The method is based on the following properties of avidin: solubility at a high (NH4)2SO4 concentration, stability and relatively low solubility in organic solvents, as well as the strongly cationic nature of the molecule. Riboflavin-binding glycoprotein may be isolated as by-product.Entities:
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Year: 1990 PMID： 2302170 PMCID： PMC1136643 DOI： 10.1042/bj2650301
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857