| Literature DB >> 23021408 |
Claudia Rengifo-Herrera1, Luis Miguel Ortega-Mora, Mercedes Gómez-Bautista, Francisco Javier García-Peña, Daniel García-Párraga, Susana Pedraza-Díaz.
Abstract
A study was conducted to investigate the presence of Cryptosporidium and Giardia in Antarctic marine mammals. A total of 270 faecal samples from different species of pinnipeds from different locations in the South Shetland Islands and Antarctic Peninsula were analysed by immunofluorescence microscopy and PCR. Cryptosporidium was detected by PCR in three samples from Southern elephant seals (Mirounga leonina) and 2 Weddell seals (Leptonychotes weddellii). However, no oocysts were observed in any of the samples by immunofluorescence microscopy. Molecular characterisation of the isolates, using the 18S rDNA, the HSP70 and the COWP loci, revealed the presence of a Cryptosporidium sp., previously reported from an Antarctic Southern elephant seal, in the elephant seals and a novel genotype in Weddell seals. Giardia could not be detected in any of the samples analysed.Entities:
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Year: 2012 PMID： 23021408 DOI： 10.1016/j.vetpar.2012.08.021
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738