AIMS: Elevated expression of DNA repair and replication genes has been reported in thick, non-fixed primary melanomas that subsequently went on to metastasize, when compared to non-recurrent primary tumours. This increased expression could contribute to the extreme resistance shown by melanoma to DNA-damaging chemotherapeutics. We have investigated the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in melanoma. METHODS AND RESULTS: We used a tissue microarray containing samples from all stages of melanomagenesis to investigate the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in a larger, more representative and readily available set of fixed primary melanomas. High expression of topoisomerase IIα (TOP2A), that relieves torsional stress during DNA replication, and XRCC5 (Ku80), required for DNA double-strand break repair, were associated with significantly worse survival. CONCLUSIONS: Two (XRCC5 and TOP2A) of seven DNA repair and replication proteins studied were prognostic for melanoma.
AIMS: Elevated expression of DNA repair and replication genes has been reported in thick, non-fixed primary melanomas that subsequently went on to metastasize, when compared to non-recurrent primary tumours. This increased expression could contribute to the extreme resistance shown by melanoma to DNA-damaging chemotherapeutics. We have investigated the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in melanoma. METHODS AND RESULTS: We used a tissue microarray containing samples from all stages of melanomagenesis to investigate the hypothesis that levels of key DNA repair and replication proteins are prognostic biomarkers in a larger, more representative and readily available set of fixed primary melanomas. High expression of topoisomerase IIα (TOP2A), that relieves torsional stress during DNA replication, and XRCC5 (Ku80), required for DNA double-strand break repair, were associated with significantly worse survival. CONCLUSIONS: Two (XRCC5 and TOP2A) of seven DNA repair and replication proteins studied were prognostic for melanoma.
Authors: Nail Fatkhutdinov; Katrin Sproesser; Clemens Krepler; Qin Liu; Patricia A Brafford; Meenhard Herlyn; Katherine M Aird; Rugang Zhang Journal: Mol Cancer Res Date: 2016-06-13 Impact factor: 5.852
Authors: Birgit Schütz; Anita Koppensteiner; David Schörghofer; Katharina Kinslechner; Gerald Timelthaler; Robert Eferl; Markus Hengstschläger; Albert Missbichler; Harald Hundsberger; Mario Mikula Journal: Sci Rep Date: 2016-11-17 Impact factor: 4.379
Authors: Liang Song; Ewan M McNeil; Ann-Marie Ritchie; Katy R Astell; Charlie Gourley; David W Melton Journal: BMC Cancer Date: 2017-12-18 Impact factor: 4.430
Authors: Stephanie B Hatch; Lonnie P Swift; Simona Caporali; Rebecca Carter; Esme J Hill; Thomas P MacGregor; Stefania D'Atri; Mark R Middleton; Peter J McHugh; Ricky A Sharma Journal: Int J Cancer Date: 2013-11-14 Impact factor: 7.396