OBJECTIVE: To evaluate DNA damage and cellular death in exfoliated buccal mucosa cells from healthy individuals following dental X-ray exposure, to compare effects of different types of radiographic techniques using the buccal cytome assay and to standardize the staining procedure. STUDY DESIGN: This study comprised 90 patients, categorized into 3 groups subjected to intraoral periapical radiography, conventional orthopantomogram and digital orthopantomogram X-ray exposure. Exfoliated oral mucosa cells were collected immediately before the X-ray and 10 days later and stained using DNA-specific stains and nonspecific DNA stains. RESULTS: The results indicated no statistically significant differences in micronucleated cells before and after dental X-ray exposure. On the other hand, X-ray exposure did increase other nuclear alterations closely related to cytotoxicity, such as karyorrhexis, pyknosis and karyolysis. CONCLUSION: These data indicate that dental radiography may not induce chromosomal damage, but it is able to promote cytotoxicity.
OBJECTIVE: To evaluate DNA damage and cellular death in exfoliated buccal mucosa cells from healthy individuals following dental X-ray exposure, to compare effects of different types of radiographic techniques using the buccal cytome assay and to standardize the staining procedure. STUDY DESIGN: This study comprised 90 patients, categorized into 3 groups subjected to intraoral periapical radiography, conventional orthopantomogram and digital orthopantomogram X-ray exposure. Exfoliated oral mucosa cells were collected immediately before the X-ray and 10 days later and stained using DNA-specific stains and nonspecific DNA stains. RESULTS: The results indicated no statistically significant differences in micronucleated cells before and after dental X-ray exposure. On the other hand, X-ray exposure did increase other nuclear alterations closely related to cytotoxicity, such as karyorrhexis, pyknosis and karyolysis. CONCLUSION: These data indicate that dental radiography may not induce chromosomal damage, but it is able to promote cytotoxicity.
Authors: D Bazyka; S C Finch; I M Ilienko; O Lyaskivska; I Dyagil; N Trotsiuk; N Gudzenko; V V Chumak; K M Walsh; J Wiemels; M P Little; L B Zablotska Journal: Environ Health Date: 2017-06-23 Impact factor: 5.984
Authors: Niels Belmans; Anne Caroline Oenning; Benjamin Salmon; Bjorn Baselet; Kevin Tabury; Stéphane Lucas; Ivo Lambrichts; Marjan Moreels; Reinhilde Jacobs; Sarah Baatout Journal: Dentomaxillofac Radiol Date: 2021-05-14 Impact factor: 3.525