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Literature DB >> 23015596

Clathrin is not required for SNX-BAR-retromer-mediated carrier formation.

Abstract

Clathrin has been implicated in retromer-mediated trafficking, but its precise function remains elusive. Given the importance of retromers for efficient endosomal sorting, we have sought to clarify the relationship between clathrin and the SNX-BAR retromer. We find that the retromer SNX-BARs do not interact directly or indirectly with clathrin. In addition, we observe that SNX-BAR-retromer tubules and carriers are not clathrin coated. Furthermore, perturbing clathrin function, by overexpressing a dominant-negative clathrin or through suppression of clathrin expression, has no detectable effect on the frequency of SNX-BAR-retromer tubulation. We propose that SNX-BAR-retromer-mediated membrane deformation and carrier formation does not require clathrin, and hence the role of clathrin in SNX-BAR-retromer function would appear to lie in pre-SNX-BAR-retromer cargo sorting.

Mesh：

Substances：

Year: 2012 PMID： 23015596 PMCID： PMC3603510 DOI： 10.1242/jcs.112904

Source DB: PubMed Journal: J Cell Sci ISSN： 0021-9533 Impact factor: 5.285

37 in total

Review 1. Tracing the retrograde route in protein trafficking.

Authors: Ludger Johannes; Vincent Popoff
Journal: Cell Date: 2008-12-26 Impact factor: 41.582

2. The BAR domain superfamily: membrane-molding macromolecules.

Authors: Adam Frost; Vinzenz M Unger; Pietro De Camilli
Journal: Cell Date: 2009-04-17 Impact factor: 41.582

3. Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8.

Authors: Anbing Shi; Lin Sun; Riju Banerjee; Michael Tobin; Yinhua Zhang; Barth D Grant
Journal: EMBO J Date: 2009-09-17 Impact factor: 11.598

4. The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex.

Authors: Emmanuel Derivery; Carla Sousa; Jérémie J Gautier; Bérangère Lombard; Damarys Loew; Alexis Gautreau
Journal: Dev Cell Date: 2009-11 Impact factor: 12.270

5. The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.

Authors: Zhi Hong; Yanrui Yang; Cheng Zhang; Yang Niu; Ke Li; Xi Zhao; Jia-Jia Liu
Journal: Cell Res Date: 2009-11-24 Impact factor: 25.617

6. Sorting nexin 8 regulates endosome-to-Golgi transport.

Authors: Anne Berit Dyve; Jonas Bergan; Audrun Utskarpen; Kirsten Sandvig
Journal: Biochem Biophys Res Commun Date: 2009-09-24 Impact factor: 3.575

7. A FAM21-containing WASH complex regulates retromer-dependent sorting.

Authors: Timothy S Gomez; Daniel D Billadeau
Journal: Dev Cell Date: 2009-11 Impact factor: 12.270

8. Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes.

Authors: Vincent Popoff; Gonzalo A Mardones; Siau-Kun Bai; Valérie Chambon; Danièle Tenza; Patricia V Burgos; Anbing Shi; Philippe Benaroch; Sylvie Urbé; Christophe Lamaze; Barth D Grant; Graça Raposo; Ludger Johannes
Journal: Traffic Date: 2009-10-05 Impact factor: 6.215

9. The phox domain of sorting nexin 5 lacks phosphatidylinositol 3-phosphate (PtdIns(3)P) specificity and preferentially binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).

Authors: Leonardus M I Koharudin; William Furey; Hao Liu; Yong-Jian Liu; Angela M Gronenborn
Journal: J Biol Chem Date: 2009-06-24 Impact factor: 5.157

10. The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network.

Authors: Thomas Wassmer; Naomi Attar; Martin Harterink; Jan R T van Weering; Colin J Traer; Jacqueline Oakley; Bruno Goud; David J Stephens; Paul Verkade; Hendrik C Korswagen; Peter J Cullen
Journal: Dev Cell Date: 2009-07 Impact factor: 12.270

16 in total

Review 1. Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease.

Authors: Frances M Brodsky; R Thomas Sosa; Joel A Ybe; Theresa J O'Halloran
Journal: Cold Spring Harb Perspect Biol Date: 2014-09-02 Impact factor: 10.005

2. Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.

Authors: Emma Sierecki; Loes M Stevers; Nichole Giles; Mark E Polinkovsky; Mehdi Moustaqil; Sergey Mureev; Wayne A Johnston; Mareike Dahmer-Heath; Dubravka Skalamera; Thomas J Gonda; Brian Gabrielli; Brett M Collins; Kirill Alexandrov; Yann Gambin
Journal: Mol Cell Proteomics Date: 2014-05-27 Impact factor: 5.911

3. Receptor-mediated Endocytosis 8 Utilizes an N-terminal Phosphoinositide-binding Motif to Regulate Endosomal Clathrin Dynamics.

Authors: Besa Xhabija; Panayiotis O Vacratsis
Journal: J Biol Chem Date: 2015-07-01 Impact factor: 5.157

Clathrin is not required for SNX-BAR-retromer-mediated carrier formation.

Review 1. Tracing the retrograde route in protein trafficking.

2. The BAR domain superfamily: membrane-molding macromolecules.

3. Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8.

4. The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex.

5. The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.

6. Sorting nexin 8 regulates endosome-to-Golgi transport.

7. A FAM21-containing WASH complex regulates retromer-dependent sorting.

8. Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes.

9. The phox domain of sorting nexin 5 lacks phosphatidylinositol 3-phosphate (PtdIns(3)P) specificity and preferentially binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).

10. The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network.

Review 1. Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease.

2. Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.

3. Receptor-mediated Endocytosis 8 Utilizes an N-terminal Phosphoinositide-binding Motif to Regulate Endosomal Clathrin Dynamics.

4. SNX-1 and RME-8 oppose the assembly of HGRS-1/ESCRT-0 degradative microdomains on endosomes.

Review 5. C. elegans as a model for membrane traffic.

Review 6. Dynamics and instabilities of lipid bilayer membrane shapes.

Review 7. Endosomal microdomains: Formation and function.

8. Sorting nexin 3 mutation impairs development and neuronal function in Caenorhabditis elegans.

9. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.

10. SNX15 links clathrin endocytosis to the PtdIns3P early endosome independently of the APPL1 endosome.