Isolation and immobilization of aroid polyphenol on magnetic nanoparticles: enhancement of potency on surface immobilization.

Five polyphenolic compounds were isolated from four aroid species [using High Performance Liquid Chromatography (HPLC)] and identified using Fourier Transform Infrared (FTIR) spectroscopy and Nuclear Magnetic Resonance (NMR) analysis. The compounds benzoic acid, caffeic acid, coumaric acid, ferulic acid and syringic acids were immobilized on magnetic nanoparticles (MNPs) using poly(ethylene glycol) (PEG) as linker polymer. The obtained PEG-MNP-polyphenol trios were characterized using transmission electron microscopy (TEM) and FTIR. Their biochemical characterization were done to find out the DPPH scavenging capacity and amount of polyphenol loaded (galic acid equivalent) in each type of MNP-polyphenol trio. To know the antioxidant activity the MNP-polyphenol trios were subjected to H(2)O(2) induced haemolysis prevention assay and syringic acid trio was found to most potent. To ensure that the MNP-polyphenol trios were magnetically active the hysteresis loop analysis was also done.