| Literature DB >> 23000963 |
Johan H van Es1, Toshiro Sato1, Marc van de Wetering1, Anna Lyubimova2, Annie Ng Yee Nee3, Alex Gregorieff4, Nobuo Sasaki1, Laura Zeinstra1, Maaike van den Born1, Jeroen Korving1, Anton C M Martens5, Nick Barker3, Alexander van Oudenaarden2, Hans Clevers1.
Abstract
Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cells form long-lived organoids (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked before tissue damage, stem cell tracing events occur. Thus, secretory progenitors exhibit plasticity by regaining stemness on damage.Entities:
Mesh：
Substances：
Year: 2012 PMID： 23000963 PMCID： PMC3789123 DOI： 10.1038/ncb2581
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824