Literature DB >> 23000821

Simple and inexpensive incorporation of 19F-tryptophan for protein NMR spectroscopy.

Peter B Crowley1, Ciara Kyne, William B Monteith.   

Abstract

Fluorine-containing amino acids are valuable probes for the biophysical characterization of proteins. Current methods for (19)F-labeled protein production involve time-consuming genetic manipulation, compromised expression systems and expensive reagents. We show that Escherichia coli BL21, the workhorse of protein production, can utilise fluoroindole for the biosynthesis of proteins containing (19)F-tryptophan.

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Year:  2012        PMID： 23000821     DOI： 10.1039/c2cc35347d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  38 in total

1.  Specific Acetylation Patterns of H2A.Z Form Transient Interactions with the BPTF Bromodomain.

Authors:  Gabriella T Perell; Neeraj K Mishra; Babu Sudhamalla; Peter D Ycas; Kabirul Islam; William C K Pomerantz
Journal:  Biochemistry       Date:  2017-08-23       Impact factor: 3.162

2.  In-cell thermodynamics and a new role for protein surfaces.

Authors:  Austin E Smith; Larry Z Zhou; Annelise H Gorensek; Michael Senske; Gary J Pielak
Journal:  Proc Natl Acad Sci U S A       Date:  2016-01-11       Impact factor: 11.205

3.  Osmotic Shock Induced Protein Destabilization in Living Cells and Its Reversal by Glycine Betaine.

Authors:  Samantha S Stadmiller; Annelise H Gorensek-Benitez; Alex J Guseman; Gary J Pielak
Journal:  J Mol Biol       Date:  2017-03-03       Impact factor: 5.469

Review 4.  A cell is more than the sum of its (dilute) parts: A brief history of quinary structure.

Authors:  Rachel D Cohen; Gary J Pielak
Journal:  Protein Sci       Date:  2017-02-13       Impact factor: 6.725

5.  Structural basis and mechanism of the unfolding-induced activation of HdeA, a bacterial acid response chaperone.

Authors:  Xing-Chi Yu; Yunfei Hu; Jienv Ding; Hongwei Li; Changwen Jin
Journal:  J Biol Chem       Date:  2018-12-20       Impact factor: 5.157

6.  (19)F Paramagnetic Relaxation Enhancement: A Valuable Tool for Distance Measurements in Proteins.

Authors:  Elena Matei; Angela M Gronenborn
Journal:  Angew Chem Int Ed Engl       Date:  2015-10-29       Impact factor: 15.336

7.  Protein-observed (19)F-NMR for fragment screening, affinity quantification and druggability assessment.

Authors:  Clifford T Gee; Keith E Arntson; Andrew K Urick; Neeraj K Mishra; Laura M L Hawk; Andrea J Wisniewski; William C K Pomerantz
Journal:  Nat Protoc       Date:  2016-07-14       Impact factor: 13.491

8.  19F Magic Angle Spinning NMR Spectroscopy and Density Functional Theory Calculations of Fluorosubstituted Tryptophans: Integrating Experiment and Theory for Accurate Determination of Chemical Shift Tensors.

Authors:  Manman Lu; Sucharita Sarkar; Mingzhang Wang; Jodi Kraus; Matthew Fritz; Caitlin M Quinn; Shi Bai; Sean T Holmes; Cecil Dybowski; Glenn P A Yap; Jochem Struppe; Ivan V Sergeyev; Werner Maas; Angela M Gronenborn; Tatyana Polenova
Journal:  J Phys Chem B       Date:  2018-05-30       Impact factor: 2.991

9.  Protein-Observed Fluorine NMR Is a Complementary Ligand Discovery Method to 1H CPMG Ligand-Observed NMR.

Authors:  Andrew K Urick; Luis Pablo Calle; Juan F Espinosa; Haitao Hu; William C K Pomerantz
Journal:  ACS Chem Biol       Date:  2016-10-05       Impact factor: 5.100

10.  Dual Screening of BPTF and Brd4 Using Protein-Observed Fluorine NMR Uncovers New Bromodomain Probe Molecules.

Authors:  Andrew K Urick; Laura M L Hawk; Melissa K Cassel; Neeraj K Mishra; Shuai Liu; Neeta Adhikari; Wei Zhang; Camila O dos Santos; Jennifer L Hall; William C K Pomerantz
Journal:  ACS Chem Biol       Date:  2015-07-28       Impact factor: 5.100
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