Literature DB >> 22990772

Transfection of Plasmodium falciparum.

Melanie Rug1, Alexander G Maier.   

Abstract

Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.

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Year:  2013        PMID： 22990772     DOI： 10.1007/978-1-62703-026-7_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  The chaperonin TRiC forms an oligomeric complex in the malaria parasite cytosol.

Authors:  Natalie J Spillman; Josh R Beck; Suresh M Ganesan; Jacquin C Niles; Daniel E Goldberg
Journal:  Cell Microbiol       Date:  2017-01-24       Impact factor: 3.715

Review 2.  Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei.

Authors:  Joachim M Matz; Taco W A Kooij
Journal:  Pathog Glob Health       Date:  2015-03-19       Impact factor: 2.894

3.  Biochemical characterization and essentiality of Plasmodium fumarate hydratase.

Authors:  Vijay Jayaraman; Arpitha Suryavanshi; Pavithra Kalale; Jyothirmai Kunala; Hemalatha Balaram
Journal:  J Biol Chem       Date:  2018-02-15       Impact factor: 5.157

4.  Spatial and temporal mapping of the PfEMP1 export pathway in Plasmodium falciparum.

Authors:  Paul J McMillan; Coralie Millet; Steven Batinovic; Mauro Maiorca; Eric Hanssen; Shannon Kenny; Rebecca A Muhle; Martin Melcher; David A Fidock; Joseph D Smith; Matthew W A Dixon; Leann Tilley
Journal:  Cell Microbiol       Date:  2013-03-14       Impact factor: 3.715

5.  Puf3 participates in ribosomal biogenesis in malaria parasites.

Authors:  Xiaoying Liang; Kevin J Hart; Gang Dong; Faiza A Siddiqui; Aswathy Sebastian; Xiaolian Li; Istvan Albert; Jun Miao; Scott E Lindner; Liwang Cui
Journal:  J Cell Sci       Date:  2018-03-26       Impact factor: 5.285

6.  Human dihydrofolate reductase influences the sensitivity of the malaria parasite Plasmodium falciparum to ketotifen - A cautionary tale in screening transgenic parasites.

Authors:  Phuong N Tran; Cameron J Tate; Melanie C Ridgway; Kevin J Saliba; Kiaran Kirk; Alexander G Maier
Journal:  Int J Parasitol Drugs Drug Resist       Date:  2016-09-22       Impact factor: 4.077

7.  Rapid Generation of Marker-Free P. falciparum Fluorescent Reporter Lines Using Modified CRISPR/Cas9 Constructs and Selection Protocol.

Authors:  Catherin Marin Mogollon; Fiona J A van Pul; Takashi Imai; Jai Ramesar; Séverine Chevalley-Maurel; Guido M de Roo; Sabrina A J Veld; Hans Kroeze; Blandine M D Franke-Fayard; Chris J Janse; Shahid M Khan
Journal:  PLoS One       Date:  2016-12-20       Impact factor: 3.240

8.  Transcriptional Profiling Defines Histone Acetylation as a Regulator of Gene Expression during Human-to-Mosquito Transmission of the Malaria Parasite Plasmodium falciparum.

Authors:  Che J Ngwa; Meike J Kiesow; Olga Papst; Lindsey M Orchard; Michael Filarsky; Alina N Rosinski; Till S Voss; Manuel Llinás; Gabriele Pradel
Journal:  Front Cell Infect Microbiol       Date:  2017-07-24       Impact factor: 5.293

9.  A seven-helix protein constitutes stress granules crucial for regulating translation during human-to-mosquito transmission of Plasmodium falciparum.

Authors:  Sandra Bennink; Andreas von Bohl; Che J Ngwa; Leonie Henschel; Andrea Kuehn; Nicole Pilch; Tim Weißbach; Alina N Rosinski; Matthias Scheuermayer; Urska Repnik; Jude M Przyborski; Allen M Minns; Lindsey M Orchard; Gareth Griffiths; Scott E Lindner; Manuel Llinás; Gabriele Pradel
Journal:  PLoS Pathog       Date:  2018-08-22       Impact factor: 6.823

10.  Successful Genetic Transfection of the Colonic Protistan Parasite Blastocystis for Reliable Expression of Ectopic Genes.

Authors:  Feng-Jun Li; Anastasios D Tsaousis; Tracy Purton; Vincent T K Chow; Cynthia Y He; Kevin S W Tan
Journal:  Sci Rep       Date:  2019-02-28       Impact factor: 4.379
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