BACKGROUND: Prognosis and appropriate treatment of head and neck squamous cell carcinoma (HNSCC) depend on the tumor type routinely derived by invasive histopathology. A promising noninvasive alternative is nonlinear optical imaging, which is capable of in vivo tissue visualization for tumor typing and grading. METHODS AND RESULTS: Thin tissue sections from 3 patients aged 56 to 60 years presenting advanced carcinoma of the hypopharynx, larynx, and left tonsil were investigated by coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and 2 photon excited fluorescence (TPEF) to study the morphochemistry of the tissues. Morphologic alterations of prognostic significance, such as cell density, nuclear to cytoplasm ratio, and keratinization as well as the underlying compositional changes during malignant transformation were determined, such as the distributions of lipids, collagen, and autofluorophors. CONCLUSIONS: Nonlinear imaging provides a noninvasive optical biopsy of the epithelial layer comparable to staining microscopy. By integration into an operational microscope, routine screening of suspicious lesions and surgical guidance can be realized.
BACKGROUND: Prognosis and appropriate treatment of head and neck squamous cell carcinoma (HNSCC) depend on the tumor type routinely derived by invasive histopathology. A promising noninvasive alternative is nonlinear optical imaging, which is capable of in vivo tissue visualization for tumor typing and grading. METHODS AND RESULTS: Thin tissue sections from 3 patients aged 56 to 60 years presenting advanced carcinoma of the hypopharynx, larynx, and left tonsil were investigated by coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and 2 photon excited fluorescence (TPEF) to study the morphochemistry of the tissues. Morphologic alterations of prognostic significance, such as cell density, nuclear to cytoplasm ratio, and keratinization as well as the underlying compositional changes during malignant transformation were determined, such as the distributions of lipids, collagen, and autofluorophors. CONCLUSIONS: Nonlinear imaging provides a noninvasive optical biopsy of the epithelial layer comparable to staining microscopy. By integration into an operational microscope, routine screening of suspicious lesions and surgical guidance can be realized.
Authors: Adam Shadfan; Hawraa Darwiche; Jesus Blanco; Ann Gillenwater; Rebecca Richards-Kortum; Tomasz S Tkaczyk Journal: Biomed Opt Express Date: 2017-02-14 Impact factor: 3.732
Authors: Stephen A Boppart; J Quincy Brown; Camile S Farah; Esther Kho; Laura Marcu; Christobel M Saunders; Henricus J C M Sterenborg Journal: J Biomed Opt Date: 2017-12 Impact factor: 3.170
Authors: Olga Chernavskaia; Sandro Heuke; Michael Vieth; Oliver Friedrich; Sebastian Schürmann; Raja Atreya; Andreas Stallmach; Markus F Neurath; Maximilian Waldner; Iver Petersen; Michael Schmitt; Thomas Bocklitz; Jürgen Popp Journal: Sci Rep Date: 2016-07-13 Impact factor: 4.379
Authors: Elen Tolstik; Liubov A Osminkina; Denis Akimov; Maksim B Gongalsky; Andrew A Kudryavtsev; Victor Yu Timoshenko; Rainer Heintzmann; Vladimir Sivakov; Jürgen Popp Journal: Int J Mol Sci Date: 2016-09-12 Impact factor: 5.923