| Literature DB >> 22987412 |
Nataša Mehle1, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, Marina Dermastia.
Abstract
The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.Mesh：
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Year: 2013 PMID： 22987412 DOI： 10.1007/978-1-62703-089-2_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745