| Literature DB >> 22987410 |
Nancy M Andrade1, Nolberto L Arismendi.
Abstract
The 4',6-diamidino-2-phenylindole (DAPI) stain technique is a simple method that was developed for confirming the presence of phytoplasmas in hand-cut or freezing microtome sections of infected tissues. DAPI binds AT-rich DNA preferentially, so that phytoplasmas, localized among phloem cells, can be visualized in a fluorescence microscope. The procedure is quick, easy to use, inexpensive, and can be used as a preliminary or quantitative method to detect or quantify phytoplasma-like bodies in infected plants.Entities:
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Year: 2013 PMID： 22987410 DOI： 10.1007/978-1-62703-089-2_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745