Literature DB >> 22986100

Wintergreen oil: a novel method in Wheatley's trichrome staining technique.

Fatmah Md Salleh1, Tengku Shahrul Anuar, Azlin Mohd Yasin, Norhayati Moktar.   

Abstract

Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique, as the reference technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 22986100     DOI： 10.1016/j.mimet.2012.08.004

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  7 in total

1.  Role of Seasonal Variation on The Prevalence and Risk Factors of Trichuriasis Among the Aboriginal Community in Malaysia.

Authors:  S A Noradilah; T S Anuar; I L Lee
Journal:  Helminthologia       Date:  2022-05-04       Impact factor: 1.176

2.  Blastocystis infection in Malaysia: evidence of waterborne and human-to-human transmissions among the Proto-Malay, Negrito and Senoi tribes of Orang Asli.

Authors:  Tengku Shahrul Anuar; Mohamed Kamel Abdul Ghani; Siti Nor Azreen; Fatmah Md Salleh; Norhayati Moktar
Journal:  Parasit Vectors       Date:  2013-02-22       Impact factor: 3.876

3.  Different clinical outcomes of Entamoeba histolytica in Malaysia: does genetic diversity exist?

Authors:  Tengku Shahrul Anuar; Hesham M Al-Mekhlafi; Mohamed Kamel Abdul Ghani; Siti Nor Azreen; Fatmah Md Salleh; Nuraffini Ghazali; Mekadina Bernadus; Norhayati Moktar
Journal:  Korean J Parasitol       Date:  2013-04-25       Impact factor: 1.341

4.  Prevalence and risk factors associated with Entamoeba histolytica/dispar/moshkovskii infection among three Orang Asli ethnic groups in Malaysia.

Authors:  Tengku Shahrul Anuar; Hesham M Al-Mekhlafi; Mohamed Kamel Abdul Ghani; Emelia Osman; Azlin Mohd Yasin; Anisah Nordin; Siti Nor Azreen; Fatmah Md Salleh; Nuraffini Ghazali; Mekadina Bernadus; Norhayati Moktar
Journal:  PLoS One       Date:  2012-10-25       Impact factor: 3.240

5.  Molecular epidemiology of giardiasis among Orang Asli in Malaysia: application of the triosephosphate isomerase gene.

Authors:  Tengku Shahrul Anuar; Siti Nor Azreen; Fatmah Md Salleh; Norhayati Moktar
Journal:  BMC Infect Dis       Date:  2014-02-12       Impact factor: 3.090

6.  Soil-transmitted helminth infections and associated risk factors in three Orang Asli tribes in Peninsular Malaysia.

Authors:  Tengku Shahrul Anuar; Fatmah Md Salleh; Norhayati Moktar
Journal:  Sci Rep       Date:  2014-02-14       Impact factor: 4.379

7.  Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. in Stool Samples.

Authors:  Nabilah Amelia Mohammad; Mohd Fahmi Mastuki; Hesham M Al-Mekhlafi; Norhayati Moktar; Tengku Shahrul Anuar
Journal:  Iran J Parasitol       Date:  2018 Jan-Mar       Impact factor: 1.012
  7 in total

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