Chao Yuan1, Mingyan Xu, Gengzhen Chen, Yucai Fu, Xiaoling Deng. 1. Department of Cell Biology and Genetics, Shantou University Medical College, Shantou University Medical College, Shantou 515041, China. yuanchao.10086@163.com
Abstract
OBJECTIVE: To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1). METHODS: Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR. RESULTS: The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects. CONCLUSION: Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.
OBJECTIVE: To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1). METHODS: Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR. RESULTS: The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects. CONCLUSION:Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.