| Literature DB >> 22983110 |
Sulaiman Ngongu Depamede, Made Sriasih, Masaharu Takahashi, Shigeo Nagashima, Suljid Jirintai, Tsutomu Nishizawa, Hiroaki Okamoto.
Abstract
One hundred sixteen rats (Rattus rattus) captured in Indonesia from 2011 to 2012 were investigated for the prevalence of hepatitis E virus (HEV)-specific antibodies and HEV RNA. Using an ELISA based on HEV genotype 4 with an ad hoc cutoff value of 0.500, 18.1 % of the rats tested positive for anti-HEV IgG. By nested RT-PCR, 14.7 % of the rats had rat HEV RNA, and none were positive for HEV genotype 1-4. A high HEV prevalence among rats was associated with lower sanitary conditions in areas with a high population density. Sixteen of the 17 HEV isolates obtained from infected rats showed >93.0 % nucleotide sequence identity within the 840-nucleotide ORF1-ORF2 sequence and were most closely related to a Vietnamese strain (85.9-87.9 % identity), while the remaining isolate differed from known rat HEV strains by 18.8-23.3 % and may belong to a novel lineage of rat HEV. These results suggest a wide distribution of rat HEV with divergent genomes.Entities:
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Year: 2012 PMID： 22983110 DOI： 10.1007/s00705-012-1462-0
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574