Cd2+ toxicity as affected by bare TiO2 nanoparticles and their bulk counterpart.

Toxicity of engineered nanoparticles has received extensive attention in recent years. However, nanoparticles always co-exist with other pollutants in natural environment. Whether there are any interactions between these classical pollutants and nanoparticles; and how these interactions may influence the environmental behavior, effects and fate of each other remain largely unclear. For this purpose, effects of bare titanium dioxide engineered nanoparticles (TiO(2)-NP) and their bulk counterpart (TiO(2)-BC) on Cd(2+) bioavailability and toxicity to the green alga Chlamydomonas reinhardtii were examined in the present study. We first investigated the kinetics and equilibrium isotherm of Cd(2+) adsorption on both particles in the algal culture medium. Pseudo-first-order adsorption kinetics was observed with equilibrium rate constant ranging from 0.19 to 0.33min(-1). Increase in Cd(2+) adsorption with its ambient concentration at equilibrium followed a single Langmuir isotherm for different concentrations of TiO(2). Furthermore, surface-area-based Cd(2+) adsorption by TiO(2)-BC was higher than that by TiO(2)-NP in most Cd(2+) concentration treatments suggesting that particle size was not the only cause for different adsorption. Both forms of TiO(2) could alleviate Cd(2+) inhibitive effects on C. reinhardtii. However, Cd(2+) toxicity and its bioaccumulation were comparable as long as its free ion concentration in ambient toxicity media was similar regardless the particle size and concentration of TiO(2). There was no TiO(2) inside the algal cells either. Therefore, it was Cd(2+) adsorption by TiO(2) which decreased its ambient free ion concentration and further its intracellular accumulation as well as toxicity.