| Literature DB >> 2297528 |
Abstract
Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3.10(-5) for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively.Entities:
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Year: 1990 PMID： 2297528 DOI： 10.1016/0167-4781(90)90029-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002