Literature DB >> 22972818

Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay.

Elisa Lazzeri1, Francesco Santoro, Marco R Oggioni, Francesco Iannelli, Gianni Pozzi.   

Abstract

A PCR-microarray assay was developed in which PCR primers and hybridization probes were designed for the 16S rRNA genes of 16 clinically relevant mycobacteria and for IS6110 of Mycobacterium tuberculosis. The assay, based on a multiplex PCR followed by hybridization with oligonucleotide probes, was tested against 16 Mycobacterium species and 70 clinical samples.

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Year:  2012        PMID： 22972818      PMCID： PMC3486262          DOI： 10.1128/JCM.02300-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

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