OBJECTIVE AND DESIGN: The pathophysiology of ischemia/reperfusion (I/R) injury is dominated by an inflammatory response. In the identification of new therapeutic agents, the role of individual cytokines may be essential. Interleukin (IL)-9 is a pleiotropic cytokine recently identified to be involved in various immune responses. In this study, the role of IL-9 in renal I/R injury was assessed. METHODS: We performed repeated direct measurements of arteriovenous IL-9 concentration differences over the reperfused graft in human kidney transplantation. RESULTS: Substantial renal IL-9 release was observed from deceased donor kidneys (P = 0.006). In contrast, living donor kidneys, which have a more favourable clinical outcome, did not release IL-9 during early reperfusion (P = 0.78). Tissue expression of IL-9 did not change upon reperfusion in both living and deceased human donor kidneys. To assess the role of IL-9 in I/R injury, an experimental study comprising IL-9 inhibition in mice undergoing renal I/R was performed. Although there was no difference in kidney function, structural damage was significantly aggravated in anti-IL-9 treated mice. CONCLUSIONS: Deceased donor grafts show a substantial IL-9 release upon reperfusion in clinical kidney transplantation. However, inhibition of IL-9 aggravated kidney damage, suggesting a regulating or minor role of IL-9 in clinical I/R injury.
OBJECTIVE AND DESIGN: The pathophysiology of ischemia/reperfusion (I/R) injury is dominated by an inflammatory response. In the identification of new therapeutic agents, the role of individual cytokines may be essential. Interleukin (IL)-9 is a pleiotropic cytokine recently identified to be involved in various immune responses. In this study, the role of IL-9 in renal I/R injury was assessed. METHODS: We performed repeated direct measurements of arteriovenousIL-9 concentration differences over the reperfused graft in human kidney transplantation. RESULTS: Substantial renal IL-9 release was observed from deceased donor kidneys (P = 0.006). In contrast, living donor kidneys, which have a more favourable clinical outcome, did not release IL-9 during early reperfusion (P = 0.78). Tissue expression of IL-9 did not change upon reperfusion in both living and deceased humandonor kidneys. To assess the role of IL-9 in I/R injury, an experimental study comprising IL-9 inhibition in mice undergoing renal I/R was performed. Although there was no difference in kidney function, structural damage was significantly aggravated in anti-IL-9 treated mice. CONCLUSIONS: Deceased donor grafts show a substantial IL-9 release upon reperfusion in clinical kidney transplantation. However, inhibition of IL-9 aggravated kidney damage, suggesting a regulating or minor role of IL-9 in clinical I/R injury.
Authors: O H Koning; R J Ploeg; J H van Bockel; M Groenewegen; F J van der Woude; G G Persijn; J Hermans Journal: Transplantation Date: 1997-06-15 Impact factor: 4.939
Authors: Li-Fan Lu; Evan F Lind; David C Gondek; Kathy A Bennett; Michael W Gleeson; Karina Pino-Lagos; Zachary A Scott; Anthony J Coyle; Jennifer L Reed; Jacques Van Snick; Terry B Strom; Xin Xiao Zheng; Randolph J Noelle Journal: Nature Date: 2006-08-20 Impact factor: 49.962
Authors: Wayel Jassem; Dicken D H Koo; Paolo Muiesan; Lucia Cerundolo; Mohamed Rela; Susan V Fuggle; Nigel D Heaton Journal: Transplantation Date: 2003-04-27 Impact factor: 4.939
Authors: D K de Vries; J H N Lindeman; D Tsikas; E de Heer; A Roos; J W de Fijter; A G Baranski; J van Pelt; A F M Schaapherder Journal: Am J Transplant Date: 2009-05-20 Impact factor: 8.086
Authors: Marina Gabriela Monteiro Carvalho Mori da Cunha; Silvia Zia; Fanny Oliveira Arcolino; Marianne Sylvia Carlon; Diego Vilibaldo Beckmann; Ney Luis Pippi; Dominguita Luhers Graça; Elena Levtchenko; Jan Deprest; Jaan Toelen Journal: PLoS One Date: 2015-08-21 Impact factor: 3.240