AIM: To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis. METHODS: Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied. RESULTS: The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5. CONCLUSION: These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
AIM: To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis. METHODS: Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied. RESULTS: The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5. CONCLUSION: These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
Authors: Z Liu; X Yang; Z Li; C McMahon; C Sizer; L Barenboim-Stapleton; V Bliskovsky; B Mock; T Ried; W B London; J Maris; J Khan; C J Thiele Journal: Cell Death Differ Date: 2011-01-21 Impact factor: 15.828
Authors: Marjan M Weiss; Antoine M Snijders; Ernst J Kuipers; Bauke Ylstra; Daniel Pinkel; Stefan G M Meuwissen; Paul J van Diest; Donna G Albertson; Gerrit A Meijer Journal: J Pathol Date: 2003-07 Impact factor: 7.996
Authors: Armand Valsesia; Donata Rimoldi; Danielle Martinet; Mark Ibberson; Paola Benaglio; Manfredo Quadroni; Patrice Waridel; Muriel Gaillard; Mireille Pidoux; Blandine Rapin; Carlo Rivolta; Ioannis Xenarios; Andrew J G Simpson; Stylianos E Antonarakis; Jacques S Beckmann; C Victor Jongeneel; Christian Iseli; Brian J Stevenson Journal: PLoS One Date: 2011-04-08 Impact factor: 3.240
Authors: Marjan M Weiss; Ernst J Kuipers; Cindy Postma; Antoine M Snijders; Daniel Pinkel; Stefan G M Meuwissen; Donna Albertson; Gerrit A Meijer Journal: Cell Oncol Date: 2004 Impact factor: 6.730