Literature DB >> 2296721

Identification of geranylgeranyl-modified proteins in HeLa cells.

C C Farnsworth1, M H Gelb, J A Glomset.   

Abstract

Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.

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Year:  1990        PMID： 2296721      PMCID： PMC3442113          DOI： 10.1126/science.2296721

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  13 in total

1.  Prenylated proteins from kidney.

Authors:  E Bruenger; H C Rilling
Journal:  Biochem Biophys Res Commun       Date:  1986-08-29       Impact factor: 3.575

2.  Defective macromolecule biosynthesis and cell-cycle progression in a mammalian cell starved for mevalonate.

Authors:  M Sinensky; J Logel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

3.  Cellular distribution of cholesterogenesis-linked, phosphoisoprenylated proteins in proliferating cells.

Authors:  L Sepp-Lorenzino; N Azrolan; P S Coleman
Journal:  FEBS Lett       Date:  1989-03-13       Impact factor: 4.124

4.  Evidence for modification of lamin B by a product of mevalonic acid.

Authors:  S L Wolda; J A Glomset
Journal:  J Biol Chem       Date:  1988-05-05       Impact factor: 5.157

5.  Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.

Authors:  R J Anderegg; R Betz; S A Carr; J W Crabb; W Duntze
Journal:  J Biol Chem       Date:  1988-12-05       Impact factor: 5.157

6.  Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans.

Authors:  W R Schafer; R Kim; R Sterne; J Thorner; S H Kim; J Rine
Journal:  Science       Date:  1989-07-28       Impact factor: 47.728

7.  All ras proteins are polyisoprenylated but only some are palmitoylated.

Authors:  J F Hancock; A I Magee; J E Childs; C J Marshall
Journal:  Cell       Date:  1989-06-30       Impact factor: 41.582

8.  Evidence for post-translational incorporation of a product of mevalonic acid into Swiss 3T3 cell proteins.

Authors:  R A Schmidt; C J Schneider; J A Glomset
Journal:  J Biol Chem       Date:  1984-08-25       Impact factor: 5.157

9.  Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases.

Authors:  S Clarke; J P Vogel; R J Deschenes; J Stock
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

10.  Isoprenoid synthesis during the cell cycle. Studies of 3-hydroxy-3-methylglutaryl-coenzyme A synthase and reductase and isoprenoid labeling in cells synchronized by centrifugal elutriation.

Authors:  W A Maltese; K M Sheridan
Journal:  J Biol Chem       Date:  1988-07-25       Impact factor: 5.157
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  47 in total

1.  Structural analysis of protein prenyl groups and associated C-terminal modifications.

Authors:  M E Whitten; K Yokoyama; D Schieltz; F Ghomashchi; D Lam; J R Yates; K Palczewski; M H Gelb
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

2.  Prenylation of mammalian Ras protein in Xenopus oocytes.

Authors:  R Kim; J Rine; S H Kim
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

3.  GGPPS, a new EGR-1 target gene, reactivates ERK 1/2 signaling through increasing Ras prenylation.

Authors:  Ning Shen; Yue Shao; Shan-Shan Lai; Long Qiao; Run-Lin Yang; Bin Xue; Fei-Yan Pan; Hua-Qun Chen; Chao-Jun Li
Journal:  Am J Pathol       Date:  2011-10-06       Impact factor: 4.307

Review 4.  Nuclear lamins.

Authors:  Thomas Dechat; Stephen A Adam; Pekka Taimen; Takeshi Shimi; Robert D Goldman
Journal:  Cold Spring Harb Perspect Biol       Date:  2010-09-08       Impact factor: 10.005

5.  A protein geranylgeranyltransferase from bovine brain: implications for protein prenylation specificity.

Authors:  K Yokoyama; G W Goodwin; F Ghomashchi; J A Glomset; M H Gelb
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

6.  Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product.

Authors:  A A Finegold; D I Johnson; C C Farnsworth; M H Gelb; S R Judd; J A Glomset; F Tamanoi
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

Review 7.  Therapeutic intervention based on protein prenylation and associated modifications.

Authors:  Michael H Gelb; Lucas Brunsveld; Christine A Hrycyna; Susan Michaelis; Fuyuhiko Tamanoi; Wesley C Van Voorhis; Herbert Waldmann
Journal:  Nat Chem Biol       Date:  2006-10       Impact factor: 15.040

8.  Suppression of yeast geranylgeranyl transferase I defect by alternative prenylation of two target GTPases, Rho1p and Cdc42p.

Authors:  Y Ohya; H Qadota; Y Anraku; J R Pringle; D Botstein
Journal:  Mol Biol Cell       Date:  1993-10       Impact factor: 4.138

Review 9.  Aberrant function of the Ras signal transduction pathway in human breast cancer.

Authors:  G J Clark; C J Der
Journal:  Breast Cancer Res Treat       Date:  1995-07       Impact factor: 4.872

10.  Changes in Protein Isoprenylation during the Growth of Suspension-Cultured Tobacco Cells.

Authors:  T. A. Morehead; B. J. Biermann; D. N. Crowell; S. K. Randall
Journal:  Plant Physiol       Date:  1995-09       Impact factor: 8.340
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