Probing lymphoma infiltration in spleen of AKR/J mice chronically exposed to electromagnetic fields for risk assessment--toward noninvasive modeling.

Using the AKR/J mouse model, the potential of Raman spectroscopy for monitoring lymphoma in predisposed subjects is demonstrated by discriminating lymphoma infiltration in spleens; the relevance of different excitation profiles is shown. Under green excitation with optimal fluorescence bleaching, stronger DNA bands, intensity variations at amide-III and phenylalanine bands, and the behavior of the 1606/1639 cm(-1) doublet correlate with tumorigenesis. Under red excitation, Raman fingerprints with multivariate models help to discriminate AKR/J-mouse histological subtypes: Lymphoblastic lymphoma (LB) is found significantly distant from both separated lymphocytic lymphoma (LL) and healthy spleen; this agrees with histology since LB has well differentiated large lymphoma cells, while LL, with smaller cells similar to normal lymphocytes, usually cannot be discriminated from normal tissue without histoimmunoassays.