Literature DB >> 22961044

Characterization of two mammalian cortical collecting duct cell lines with hopping probe ion conductance microscopy.

Xuewei Chen1, Hui Zhu, Xiao Liu, Hujie Lu, Ying Li, Jing Wang, Hongtao Liu, Jianning Zhang, Qiang Ma, Yanjun Zhang.   

Abstract

We morphologically and physiologically characterized Madin-Darby canine kidney (MDCK) cell and mouse principal cell of kidney in cortical collecting duct (mpkCCD) via hopping probe ion conductance microscopy, transepithelial electrical resistance (TEER) measurements, and single-channel recordings. The specific membrane structures of microvilli and tight junctions were clearly observed in MDCK and mpkCCD cell monolayers. The electrophysiological functions of epithelial Na(+) channel in MDCK and mpkCCD cells were further characterized by measuring amiloride-sensitive TEER values for the whole-cell monolayer and detecting the ion channel activities with patch clamping. Our results provide more morphological and functional information to help better utilize these two mammalian CCD cell lines for mechanism studies of sodium absorption and reabsorption in the distal nephron.

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Year:  2012        PMID： 22961044     DOI： 10.1007/s00232-012-9495-6

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  28 in total

1.  Comparison of scanning ion conductance microscopy with atomic force microscopy for cell imaging.

Authors:  Johannes Rheinlaender; Nicholas A Geisse; Roger Proksch; Tilman E Schäffer
Journal:  Langmuir       Date:  2010-12-15       Impact factor: 3.882

2.  Investigation of morphological and functional changes during neuronal differentiation of PC12 cells by combined hopping probe ion conductance microscopy and patch-clamp technique.

Authors:  Xi Yang; Xiao Liu; Xiaofan Zhang; Hujie Lu; Jianning Zhang; Yanjun Zhang
Journal:  Ultramicroscopy       Date:  2011-06-01       Impact factor: 2.689

Review 3.  Non-genomic actions of aldosterone: from receptors and signals to membrane targets.

Authors:  Ruth Dooley; Brian J Harvey; Warren Thomas
Journal:  Mol Cell Endocrinol       Date:  2011-07-27       Impact factor: 4.102

4.  Real-time investigation of acute toxicity of ZnO nanoparticles on human lung epithelia with hopping probe ion conductance microscopy.

Authors:  Xi Yang; Xiao Liu; Hujie Lu; Xiaofan Zhang; Liying Ma; Ruiling Gao; Yanjun Zhang
Journal:  Chem Res Toxicol       Date:  2012-01-06       Impact factor: 3.739

5.  Claudin-2, a component of the tight junction, forms a paracellular water channel.

Authors:  Rita Rosenthal; Susanne Milatz; Susanne M Krug; Beibei Oelrich; Jörg-Dieter Schulzke; Salah Amasheh; Dorothee Günzel; Michael Fromm
Journal:  J Cell Sci       Date:  2010-05-11       Impact factor: 5.285

6.  Basolateral P2X4-like receptors regulate the extracellular ATP-stimulated epithelial Na+ channel activity in renal epithelia.

Authors:  Yanjun Zhang; Daniel Sanchez; Julia Gorelik; David Klenerman; Max Lab; Christopher Edwards; Yuri Korchev
Journal:  Am J Physiol Renal Physiol       Date:  2007-03-13

7.  Hypotonicity-induced reduction of aquaporin-2 transcription in mpkCCD cells is independent of the tonicity responsive element, vasopressin, and cAMP.

Authors:  Marleen L A Kortenoeven; Michiel van den Brand; Jack F M Wetzels; Peter M T Deen
Journal:  J Biol Chem       Date:  2011-02-15       Impact factor: 5.157

8.  Nanoscale live-cell imaging using hopping probe ion conductance microscopy.

Authors:  Pavel Novak; Chao Li; Andrew I Shevchuk; Ruben Stepanyan; Matthew Caldwell; Simon Hughes; Trevor G Smart; Julia Gorelik; Victor P Ostanin; Max J Lab; Guy W J Moss; Gregory I Frolenkov; David Klenerman; Yuri E Korchev
Journal:  Nat Methods       Date:  2009-03-01       Impact factor: 28.547

9.  The use of scanning ion conductance microscopy to image A6 cells.

Authors:  Julia Gorelik; Yanjun Zhang; Andrew I Shevchuk; Gregory I Frolenkov; Daniel Sánchez; Max J Lab; Igor Vodyanoy; Christopher R W Edwards; David Klenerman; Yuri E Korchev
Journal:  Mol Cell Endocrinol       Date:  2004-03-31       Impact factor: 4.102

10.  Intact cytoskeleton is required for small G protein dependent activation of the epithelial Na+ channel.

Authors:  Alexey V Karpushev; Daria V Ilatovskaya; Tengis S Pavlov; Yuri A Negulyaev; Alexander Staruschenko
Journal:  PLoS One       Date:  2010-01-21       Impact factor: 3.240
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  1 in total

1.  Phosphoinositide 3-kinase pathway mediates early aldosterone action on morphology and epithelial sodium channel in mammalian renal epithelia.

Authors:  Yuan Zhou; Xuewei Chen; Xiao Liu; Hujie Lu; Ying Li; Hui Zhu; Gaihong An; Na Zhang; Jianning Zhang; Qiang Ma; Yanjun Zhang
Journal:  J Membr Biol       Date:  2014-04-11       Impact factor: 1.843
  1 in total

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