AIMS: The complement system, and especially C5a, plays an important role in the pathophysiology of renal diseases and post-transplant renal injury. The two receptors for C5a are C5a receptor (C5aR) and C5a-like-receptor-2 (C5L2). Only renal C5aR expression has been reported, although exact localization and alterations in expression after transplantation are unknown. MATERIALS AND RESULTS: Renal C5aR and C5L2 expression and localization were analyzed immunohistochemically. C5aR and C5L2 expression was analyzed in human kidney biopsies obtained from living donors and patients suffering from acute tubular necrosis, acute cellular and vascular rejection or IF/TA. C5aR was expressed in the thick ascending limb of Henle's loop and first part of the distal convoluted tubule (DCT). Under inflammatory conditions, C5aR was de novo expressed in proximal tubuli. C5L2 was expressed in the kidney and localized to DCT1, DCT2 and connecting tubule. Persistent distal tubular expression of both receptors was demonstrated after renal transplantation. CONCLUSIONS: This study shows distinct renal expression patterns for C5aR and C5L2. Our findings suggest a functional role for renal C5L2 rather than being a C5a decoy receptor. Future studies focusing on renal C5a-C5aR interaction should take differential C5aR and C5L2 expression into account, alongside abundant C5aR expression on infiltrating cells.
AIMS: The complement system, and especially C5a, plays an important role in the pathophysiology of renal diseases and post-transplant renal injury. The two receptors for C5a are C5a receptor (C5aR) and C5a-like-receptor-2 (C5L2). Only renal C5aR expression has been reported, although exact localization and alterations in expression after transplantation are unknown. MATERIALS AND RESULTS: Renal C5aR and C5L2 expression and localization were analyzed immunohistochemically. C5aR and C5L2 expression was analyzed in human kidney biopsies obtained from living donors and patients suffering from acute tubular necrosis, acute cellular and vascular rejection or IF/TA. C5aR was expressed in the thick ascending limb of Henle's loop and first part of the distal convoluted tubule (DCT). Under inflammatory conditions, C5aR was de novo expressed in proximal tubuli. C5L2 was expressed in the kidney and localized to DCT1, DCT2 and connecting tubule. Persistent distal tubular expression of both receptors was demonstrated after renal transplantation. CONCLUSIONS: This study shows distinct renal expression patterns for C5aR and C5L2. Our findings suggest a functional role for renal C5L2 rather than being a C5a decoy receptor. Future studies focusing on renal C5a-C5aR interaction should take differential C5aR and C5L2 expression into account, alongside abundant C5aR expression on infiltrating cells.
Authors: Bernd Schröppel; Peter S Heeger; Heather Thiessen-Philbrook; Isaac E Hall; Mona D Doshi; Francis L Weng; Peter P Reese; Chirag R Parikh Journal: Transplantation Date: 2019-01 Impact factor: 4.939
Authors: Giuseppe Castellano; Rossana Franzin; Fabio Sallustio; Alessandra Stasi; Barbara Banelli; Massimo Romani; Giuseppe De Palma; Giuseppe Lucarelli; Chiara Divella; Michele Battaglia; Antonio Crovace; Francesco Staffieri; Giuseppe Grandaliano; Giovanni Stallone; Pasquale Ditonno; Paolo Cravedi; Vincenzo Cantaluppi; Loreto Gesualdo Journal: Aging (Albany NY) Date: 2019-07-08 Impact factor: 5.682
Authors: Carlos Arias-Cabrales; Eva Rodriguez-Garcia; Javier Gimeno; David Benito; María José Pérez-Sáez; Dolores Redondo-Pachón; Anna Buxeda; Carla Burballa; Marta Crespo; Marta Riera; Julio Pascual Journal: J Clin Med Date: 2021-03-02 Impact factor: 4.241