ETHNOPHARMACOLOGICAL RELEVANCE: Seeds of Centratherum anthelminticum (Asteraceae) have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders. Folk medicine from Rayalaseema (Andhra Pradesh, India) reported wide spread usage in diabetes. AIM OF THE STUDY: To investigate the hypoglycemic properties and mechanism of the methanolic fraction of C. anthelminticum seeds (CAMFs) on mouse β-TC6 pancreatic cell line and streptozotocin (STZ)-induced diabetic rat models. MATERIALS AND METHODS: We investigated the crude methanolic fraction of C. anthelminticum seeds (CAMFs) on β-TC6 cell line and confirmed its effects on type 1 and type 2 diabetic rats to understand its mechanism in managing diabetes mellitus. CAMFs were initially tested on β-TC6 cells for cytotoxicity, 2-NBDG glucose uptake, insulin secretion and glucose transporter (GLUT-1, 2 and 4) protein expression. Furthermore, streptozotocin (STZ)-induced type 1 diabetic and STZ-nicotinamide-induced type 2 diabetic rats were intraperitoneally (i.p) injected or administered orally with CAMFs daily for 28 days. The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated. RESULTS: In cell line studies, CAMFs showed non-cytotoxic effect on β-TC6 cell proliferation compared to untreated control cells at 50 μg/ml. CAMFs increased glucose uptake and insulin secretion dose-dependently by up-regulating GLUT-2 and GLUT-4 expression in these cells. Further in vivo studies on streptozotocin induced diabetic rat models revealed that CAMFs significantly reduced hyperglycemia by augmenting insulin secretion in type 2 diabetic rats. However, CAMFs displayed less significant effects on type 1 diabetic rats. CONCLUSIONS: CAMFs demonstrated anti-diabetic potential on β-TC6 cells and type 2 diabetic rat model, plausibly through enhancing glucose uptake and insulin secretion.
ETHNOPHARMACOLOGICAL RELEVANCE: Seeds of Centratherum anthelminticum (Asteraceae) have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders. Folk medicine from Rayalaseema (Andhra Pradesh, India) reported wide spread usage in diabetes. AIM OF THE STUDY: To investigate the hypoglycemic properties and mechanism of the methanolic fraction of C. anthelminticum seeds (CAMFs) on mouse β-TC6 pancreatic cell line and streptozotocin (STZ)-induced diabeticrat models. MATERIALS AND METHODS: We investigated the crude methanolic fraction of C. anthelminticum seeds (CAMFs) on β-TC6 cell line and confirmed its effects on type 1 and type 2 diabeticrats to understand its mechanism in managing diabetes mellitus. CAMFs were initially tested on β-TC6 cells for cytotoxicity, 2-NBDGglucose uptake, insulin secretion and glucose transporter (GLUT-1, 2 and 4) protein expression. Furthermore, streptozotocin (STZ)-induced type 1 diabetic and STZ-nicotinamide-induced type 2 diabeticrats were intraperitoneally (i.p) injected or administered orally with CAMFs daily for 28 days. The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated. RESULTS: In cell line studies, CAMFs showed non-cytotoxic effect on β-TC6 cell proliferation compared to untreated control cells at 50 μg/ml. CAMFs increased glucose uptake and insulin secretion dose-dependently by up-regulating GLUT-2 and GLUT-4 expression in these cells. Further in vivo studies on streptozotocin induced diabeticrat models revealed that CAMFs significantly reduced hyperglycemia by augmenting insulin secretion in type 2 diabeticrats. However, CAMFs displayed less significant effects on type 1 diabeticrats. CONCLUSIONS: CAMFs demonstrated anti-diabetic potential on β-TC6 cells and type 2 diabeticrat model, plausibly through enhancing glucose uptake and insulin secretion.
Authors: Chung Yeng Looi; Bushra Moharram; Mohammadjavad Paydar; Yi Li Wong; Kok Hoong Leong; Khalit Mohamad; Aditya Arya; Won Fen Wong; Mohd Rais Mustafa Journal: BMC Complement Altern Med Date: 2013-07-10 Impact factor: 3.659
Authors: Klinsmann Carolo Dos Santos; Bianca Guerra Bueno; Luana Ferreira Pereira; Fabiane Valentini Francisqueti; Mariana Gobbo Braz; Lahis Fernandes Bincoleto; Lilian Xavier da Silva; Ana Lúcia A Ferreira; Ana Cláudia de Melo Stevanato Nakamune; C-Y Oliver Chen; Jeffrey B Blumberg; Camila Renata Corrêa Journal: Evid Based Complement Alternat Med Date: 2017-07-20 Impact factor: 2.629