OBJECTIVES: Hepatitis B virus (HBV) and HIV-1 share similar transmission routes, therefore, coinfection with both viruses is frequently observed. In HIV-1-infected patients reduced interleukin 21 (IL-21) serum levels have been reported, which may impair virus-specific CD8 T-cell responses. DESIGN: The HBV-specific CD8 T cells in patients with and without HIV-1 coinfection were analyzed cross-sectionally and it was tested whether addition of IL-21 in vitro augments HBV-specific CD8 T-cell responses. METHODS: Patients with persistent HBV monoinfection as well as HIV-1-positive patients with persistent or resolved HBV-infection were studied. The IL-21 serum levels were determined by ELISA and the HBV-specific CD8 T-cell response was determined after antigen-specific expansion by intracellular staining of interferon γ. RESULTS: The HBV-specific CD8 T-cell response was significantly higher in HIV-1-negative patients compared with HIV-1-positive patients. Interestingly, within the HIV-1-positive group the magnitude of the response did not differ between patients with chronic or resolved HBV-infection. The IL-21 serum levels were significantly lower in the HIV-1-positive group. Importantly, addition of IL-21 in vitro significantly increased the HBV-specific CD8 T-cell response in HIV-1-positive patients, whereas there was no beneficial effect of IL-21 on cells from HBV-monoinfected patients. CONCLUSION: HIV-1 coinfection is associated with a decreased HBV-specific CD8 T-cell response that can be partially rescued in vitro by addition of IL-21.
OBJECTIVES:Hepatitis B virus (HBV) and HIV-1 share similar transmission routes, therefore, coinfection with both viruses is frequently observed. In HIV-1-infectedpatients reduced interleukin 21 (IL-21) serum levels have been reported, which may impair virus-specific CD8 T-cell responses. DESIGN: The HBV-specific CD8 T cells in patients with and without HIV-1 coinfection were analyzed cross-sectionally and it was tested whether addition of IL-21 in vitro augments HBV-specific CD8 T-cell responses. METHODS:Patients with persistent HBV monoinfection as well as HIV-1-positivepatients with persistent or resolved HBV-infection were studied. The IL-21 serum levels were determined by ELISA and the HBV-specific CD8 T-cell response was determined after antigen-specific expansion by intracellular staining of interferon γ. RESULTS: The HBV-specific CD8 T-cell response was significantly higher in HIV-1-negative patients compared with HIV-1-positivepatients. Interestingly, within the HIV-1-positive group the magnitude of the response did not differ between patients with chronic or resolved HBV-infection. The IL-21 serum levels were significantly lower in the HIV-1-positive group. Importantly, addition of IL-21 in vitro significantly increased the HBV-specific CD8 T-cell response in HIV-1-positivepatients, whereas there was no beneficial effect of IL-21 on cells from HBV-monoinfected patients. CONCLUSION:HIV-1 coinfection is associated with a decreased HBV-specific CD8 T-cell response that can be partially rescued in vitro by addition of IL-21.
Authors: Xue-Ping Gao; Yang Zhou; Xin-Chun Zheng; Xuan Yi; Li-Bo Tang; Jin-Lin Hou; Yong-Yin Li Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2017-11-20