OBJECTIVE: The objective of the study was to determine the effect of statins on lipopolysaccharide (LPS)-induced inflammatory response in a mouse model of preterm birth (PTB). STUDY DESIGN: Day 15 CD1 mice were randomly allocated to intraperitoneal LPS injection (100 μg) or control. Mice in the LPS group were pretreated, 16 and 2 hours prior, with pravastatin (10 μg/g), simvastatin (10 μg/g), or vehicle control. Animals were sacrificed 6 hours after LPS. Cytokine messenger ribonucleic acid (mRNA) expression in the uterus and cervix, and concentrations in the maternal serum and amniotic fluid (AF) were determined. RESULTS: Pravastatin reduced interleukin (IL)-1β and IL-6 mRNA expression in the uterus and cervix, respectively, and serum IL-1β and granulocyte-macrophage colony-stimulating factor (GM-CSF) concentrations. Simvastatin reduced IL-1β and IL-6 mRNA expressions in the uterus, IL-6 and tumor necrosis factor alpha (TNF-α) in the cervix, and IL-1β, IL-2, IL-12p70, IL-13, TNF-α, GM-CSF, and interferon-γ concentrations in the serum and IL-6 in AF. CONCLUSION: Statins reduce the LPS-induced inflammatory responses in a mouse model of PTB.
OBJECTIVE: The objective of the study was to determine the effect of statins on lipopolysaccharide (LPS)-induced inflammatory response in a mouse model of preterm birth (PTB). STUDY DESIGN: Day 15 CD1mice were randomly allocated to intraperitoneal LPS injection (100 μg) or control. Mice in the LPS group were pretreated, 16 and 2 hours prior, with pravastatin (10 μg/g), simvastatin (10 μg/g), or vehicle control. Animals were sacrificed 6 hours after LPS. Cytokine messenger ribonucleic acid (mRNA) expression in the uterus and cervix, and concentrations in the maternal serum and amniotic fluid (AF) were determined. RESULTS:Pravastatin reduced interleukin (IL)-1β and IL-6 mRNA expression in the uterus and cervix, respectively, and serum IL-1β and granulocyte-macrophage colony-stimulating factor (GM-CSF) concentrations. Simvastatin reduced IL-1β and IL-6 mRNA expressions in the uterus, IL-6 and tumor necrosis factor alpha (TNF-α) in the cervix, and IL-1β, IL-2, IL-12p70, IL-13, TNF-α, GM-CSF, and interferon-γ concentrations in the serum and IL-6 in AF. CONCLUSION: Statins reduce the LPS-induced inflammatory responses in a mouse model of PTB.
Authors: Matthew Josiah Allen-Daniels; Myrna G Serrano; Lindsey P Pflugner; Jennifer M Fettweis; Melissa A Prestosa; Vishal N Koparde; J Paul Brooks; Jerome F Strauss; Roberto Romero; Tinnakorn Chaiworapongsa; David A Eschenbach; Gregory A Buck; Kimberly K Jefferson Journal: Am J Obstet Gynecol Date: 2015-01-28 Impact factor: 8.661
Authors: Nicoletta Di Simone; Fiorella Di Nicuolo; Riccardo Marana; Roberta Castellani; Francesco Ria; Manuela Veglia; Giovanni Scambia; Daniel Surbek; Eytan Barnea; Martin Mueller Journal: PLoS One Date: 2017-07-12 Impact factor: 3.240