Characterization of "Candidatus Nebulobacter yamunensis" from the cytoplasm of Euplotes aediculatus (Ciliophora, Spirotrichea) and emended description of the family Francisellaceae.

Our knowledge of ciliate endosymbionts occurrence and diversity greatly expanded in the last decades, due to the development of characterization methods for uncultivable bacteria. Symbionts related to human pathogens such as rickettsiae and francisellae have been detected inside the cytoplasm of different ciliate species. In the present work, we have characterized a novel Francisella-related bacterium inside the rich prokaryotic community harbored by a population of Euplotes aediculatus (Ciliophora, Spirotrichea). Following the "Full-Cycle rRNA Approach" we obtained the almost full-length 16S rRNA gene sequence of this bacterium, and developed probes for diagnostic fluorescence in situ hybridizations. Attempts to culture the endosymbiont outside of its host failed. We classified this novel organism in a new taxon for which we propose the name "Candidatus Nebulobacter yamunensis". In order to investigate its evolutionary relationships, we have also performed phylogenetic analyses on the class Gammaproteobacteria and the order Thiotrichales, which include the monogeneric family Francisellaceae. We found highly supported evidences for the establishment of a new monophyletic taxon including Francisella species, other organisms currently incertae sedis, and "Candidatus Nebulobacter yamunensis". These organisms form a clade sharing a signature sequence not present in other Thiotrichales bacteria. Moreover, most of them have developed an intracellular life cycle inside eukaryotic organisms. We emended the original description of family Francisellaceae in order to encompass all members of the described clade.