AIMS: To study the importance of IκBα in NF-κB signal transduction, we analysed the IκBα expression in monocytes from Sjögren's syndrome (SS) patients versus healthy controls. METHODS: Monocytes were obtained from the peripheral blood of 30 SS patients and 23 healthy subjects. IκBα expression was studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA). RESULTS: Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IκBα, and in all the Sjögren's syndrome cases examined, serum IκBα levels were significantly decreased in comparison with controls. CONCLUSIONS: Our findings clearly demonstrate changes in the levels of IκBα in SS monocytes, suggesting that the attenuated expression of IκBα could contribute to the deregulation of NF-κB pathways in the SS pathogenesis. Decreased expression of IκBα may specifically amplify cytokines production and inflammatory response linked to Sjögren's syndrome.
AIMS: To study the importance of IκBα in NF-κB signal transduction, we analysed the IκBα expression in monocytes from Sjögren's syndrome (SS) patients versus healthy controls. METHODS: Monocytes were obtained from the peripheral blood of 30 SS patients and 23 healthy subjects. IκBα expression was studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA). RESULTS: Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IκBα, and in all the Sjögren's syndrome cases examined, serum IκBα levels were significantly decreased in comparison with controls. CONCLUSIONS: Our findings clearly demonstrate changes in the levels of IκBα in SS monocytes, suggesting that the attenuated expression of IκBα could contribute to the deregulation of NF-κB pathways in the SS pathogenesis. Decreased expression of IκBα may specifically amplify cytokines production and inflammatory response linked to Sjögren's syndrome.
Authors: Ana P Lopes; Cornelis P J Bekker; Maarten R Hillen; Sofie L M Blokland; Anneline C Hinrichs; Aridaman Pandit; Aike A Kruize; Timothy R D J Radstake; Joel A G van Roon Journal: Front Immunol Date: 2021-08-03 Impact factor: 7.561
Authors: Yi-fan Huang; Qian Cheng; Chun-miao Jiang; Shu An; Lan Xiao; Yong-chao Gou; Wen-jing Yu; Lei Lei; Qian-ming Chen; Yating Wang; Jun Wang Journal: Clin Dev Immunol Date: 2013-07-09
Authors: Adrienne E G Williams; Kevin Choi; Annie L Chan; Yun Jong Lee; Westley H Reeves; Michael R Bubb; Carol M Stewart; Seunghee Cha Journal: Arthritis Res Ther Date: 2016-05-03 Impact factor: 5.156