| Literature DB >> 22932951 |
Marthe T C Walvoort1, Wouter W Kallemeijn, Lianne I Willems, Martin D Witte, Johannes M F G Aerts, Gijsbert A van der Marel, Jeroen D C Codée, Herman S Overkleeft.
Abstract
The potency of 2-deoxy-2-fluoroglycosides in activity-based profiling of human acid β-glucosidase is drastically improved by introducing an N-phenyl trifluoroacetimidate leaving group at the anomeric center. Protonation by the general acid-base catalyst in the active site turned out to be a prerequisite, making the imidate probe a genuine mechanism-based glycosidase inactivator.Entities:
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Year: 2012 PMID： 22932951 DOI： 10.1039/c2cc35653h
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222