OBJECTIVE: To measure the effect labor exerts on fatty acid (FA) oxidation in term human placentas, and to compare enzymes expression and activity between placenta and liver. METHODS: Placental samples were collected: (a) scheduled non-labored cesarean section and (b) normal vaginal delivery at or beyond 37 weeks. Long and medium-chain FA oxidation were measured using (3)H-labeled FA, ATP concentration was measured via commercial kit. Activity and expression levels of 11 FA enzymes were measured and results compared to both human and mouse liver. RESULTS: Placentas undergoing labor had significantly decreased palmitate oxidation and ATP levels. Octanoic acid oxidation was 10-fold higher than palmitic acid oxidation. No difference in expression or activity level was detected between the groups. CONCLUSION: Term human placentas express all the enzymes required to oxidize FA, at a rate 20-fold lower than liver. FA Oxidation is not likely an important placental energy source during labor. Further work is needed to determine the functionality of this pathway in placenta.
OBJECTIVE: To measure the effect labor exerts on fatty acid (FA) oxidation in term human placentas, and to compare enzymes expression and activity between placenta and liver. METHODS: Placental samples were collected: (a) scheduled non-labored cesarean section and (b) normal vaginal delivery at or beyond 37 weeks. Long and medium-chain FA oxidation were measured using (3)H-labeled FA, ATP concentration was measured via commercial kit. Activity and expression levels of 11 FA enzymes were measured and results compared to both human and mouse liver. RESULTS: Placentas undergoing labor had significantly decreased palmitate oxidation and ATP levels. Octanoic acid oxidation was 10-fold higher than palmitic acid oxidation. No difference in expression or activity level was detected between the groups. CONCLUSION: Term human placentas express all the enzymes required to oxidize FA, at a rate 20-fold lower than liver. FA Oxidation is not likely an important placental energy source during labor. Further work is needed to determine the functionality of this pathway in placenta.
Authors: Elvira Larqué; Hans Demmelmair; Alfonso Gil-Sánchez; María T Prieto-Sánchez; José E Blanco; Ana Pagán; Fabienne L Faber; Salvador Zamora; Juan José Parrilla; Berthold Koletzko Journal: Am J Clin Nutr Date: 2011-05-11 Impact factor: 7.045
Authors: Michael D Wolfe; Lu-Te Chuang; William F Rayburn; Pei-Chi Wen; Dorothy J VanderJagt; Robert H Glew Journal: J Matern Fetal Neonatal Med Date: 2012-04-24
Authors: M He; S L Rutledge; D R Kelly; C A Palmer; G Murdoch; N Majumder; R D Nicholls; Z Pei; P A Watkins; J Vockley Journal: Am J Hum Genet Date: 2007-06-04 Impact factor: 11.025
Authors: Eric S Goetzman; John F Alcorn; Sivakama S Bharathi; Radha Uppala; Kevin J McHugh; Beata Kosmider; Rimei Chen; Yi Y Zuo; Megan E Beck; Richard W McKinney; Helen Skilling; Kristen R Suhrie; Anuradha Karunanidhi; Renita Yeasted; Chikara Otsubo; Bryon Ellis; Yulia Y Tyurina; Valerian E Kagan; Rama K Mallampalli; Jerry Vockley Journal: J Biol Chem Date: 2014-03-03 Impact factor: 5.157