| Literature DB >> 2292793 |
Abstract
We consider the imaging in scanning microscopes employing point and slit-shaped detectors in both the bright-field and fluorescent mode. In particular we consider the three-dimensional aspects of the imaging and show inter alia that acceptable, albeit asymmetrical, images result from a slit detector system at low levels of defocus. The situation becomes worse as the defocus is increased although acceptable extended-focus and isometric images are still possible in some cases.Mesh:
Year: 1990 PMID: 2292793 DOI: 10.1111/j.1365-2818.1990.tb03053.x
Source DB: PubMed Journal: J Microsc ISSN: 0022-2720 Impact factor: 1.758