Literature DB >> 2292596

Purification and characterization of soluble human IL-6 receptor expressed in CHO cells.

K Yasukawa1, T Saito, T Fukunaga, Y Sekimori, Y Koishihara, H Fukui, Y Ohsugi, T Matsuda, H Yawata, T Hirano.   

Abstract

An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensitivity of M1 cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml.

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Year:  1990        PMID： 2292596     DOI： 10.1093/oxfordjournals.jbchem.a123261

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  32 in total

1.  Structure of the extracellular domains of the human interleukin-6 receptor alpha -chain.

Authors:  J N Varghese; R L Moritz; M-Z Lou; A Van Donkelaar; H Ji; N Ivancic; K M Branson; N E Hall; R J Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-02       Impact factor: 11.205

2.  Functional inhibition of hematopoietic and neurotrophic cytokines by blocking the interleukin 6 signal transducer gp130.

Authors:  T Taga; M Narazaki; K Yasukawa; T Saito; D Miki; M Hamaguchi; S Davis; M Shoyab; G D Yancopoulos; T Kishimoto
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-15       Impact factor: 11.205

3.  Intercellular adhesion molecule-1 on synovial cells attenuated interleukin-6-induced inhibition of osteoclastogenesis induced by receptor activator for nuclear factor κB ligand.

Authors:  M Suzuki; M Hashizume; H Yoshida; M Shiina; M Mihara
Journal:  Clin Exp Immunol       Date:  2010-10-29       Impact factor: 4.330

4.  Engineering human interleukin-6 to obtain variants with strongly enhanced bioactivity.

Authors:  C Toniatti; A Cabibbo; E Sporena; A L Salvati; M Cerretani; S Serafini; A Lahm; R Cortese; G Ciliberto
Journal:  EMBO J       Date:  1996-06-03       Impact factor: 11.598

Review 5.  Pharmacokinetic studies with recombinant cytokines. Scientific issues and practical considerations.

Authors:  S C Piscitelli; W G Reiss; W D Figg; W P Petros
Journal:  Clin Pharmacokinet       Date:  1997-05       Impact factor: 6.447

6.  Expression of the interleukin 6 receptor in primary renal cell carcinoma.

Authors:  V Costes; J Liautard; M C Picot; M Robert; N Lequeux; J Brochier; P Baldet; J F Rossi
Journal:  J Clin Pathol       Date:  1997-10       Impact factor: 3.411

7.  Critical cytoplasmic region of the interleukin 6 signal transducer gp130 is conserved in the cytokine receptor family.

Authors:  M Murakami; M Narazaki; M Hibi; H Yawata; K Yasukawa; M Hamaguchi; T Taga; T Kishimoto
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

8.  Expansion of human NOD/SCID-repopulating cells by stem cell factor, Flk2/Flt3 ligand, thrombopoietin, IL-6, and soluble IL-6 receptor.

Authors:  T Ueda; K Tsuji; H Yoshino; Y Ebihara; H Yagasaki; H Hisakawa; T Mitsui; A Manabe; R Tanaka; K Kobayashi; M Ito; K Yasukawa; T Nakahata
Journal:  J Clin Invest       Date:  2000-04       Impact factor: 14.808

9.  Serum soluble interleukin 6 (IL-6) receptor and IL-6/soluble IL-6 receptor complex in systemic juvenile rheumatoid arthritis.

Authors:  F De Benedetti; M Massa; P Pignatti; S Albani; D Novick; A Martini
Journal:  J Clin Invest       Date:  1994-05       Impact factor: 14.808

10.  Role of the C-terminus in the activity, conformation, and stability of interleukin-6.

Authors:  L D Ward; A Hammacher; J G Zhang; J Weinstock; K Yasukawa; C J Morton; R S Norton; R J Simpson
Journal:  Protein Sci       Date:  1993-09       Impact factor: 6.725
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