Chaodong Liu1, Xian-Zhong Wang, Xin-Bo Sun. 1. Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. chaodongliu7@163.com
Abstract
BACKGROUND AND AIMS: The prevalence of recurrent miscarriage (RM) is about 1-3% of women; the pathogenesis of RM is not fully understood yet. This study aims to assess the sperm antigen specific regulatory T cells (Treg) in women with RM. METHODS: A group of women with RM was recruited into this study. The sperm antigen was extracted from the semen samples of each woman's husband. The sperm antigen specific T cell response was assessed by flow cytometry. RESULTS: Low frequency of sperm specific Tregs and high frequency of T helper (Th)1 cells were detected in RM women as compared with women without RM. The sperm specific Tregs in RM women expressed less Ubc13. Knockdown of Ubc13 from Tregs converted the Tregs to effector T cells. CONCLUSIONS: Immune deregulation may play an important role in RM.
BACKGROUND AND AIMS: The prevalence of recurrent miscarriage (RM) is about 1-3% of women; the pathogenesis of RM is not fully understood yet. This study aims to assess the sperm antigen specific regulatory T cells (Treg) in women with RM. METHODS: A group of women with RM was recruited into this study. The sperm antigen was extracted from the semen samples of each woman's husband. The sperm antigen specific T cell response was assessed by flow cytometry. RESULTS: Low frequency of sperm specific Tregs and high frequency of T helper (Th)1 cells were detected in RM women as compared with women without RM. The sperm specific Tregs in RM women expressed less Ubc13. Knockdown of Ubc13 from Tregs converted the Tregs to effector T cells. CONCLUSIONS: Immune deregulation may play an important role in RM.
Authors: Sarah A Robertson; Ella S Green; Alison S Care; Lachlan M Moldenhauer; Jelmer R Prins; M Louise Hull; Simon C Barry; Gustaaf Dekker Journal: Front Immunol Date: 2019-03-21 Impact factor: 7.561