| Literature DB >> 22924646 |
Wei Shen1, Huimin Deng, Zhiqiang Gao.
Abstract
A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).Mesh：
Substances：
Year: 2012 PMID： 22924646 DOI： 10.1021/ja306265n
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419