| Literature DB >> 22921483 |
Kathryn Oostdik1, Julie French, Donald Yet, Briana Smalling, Craig Nolde, Peter M Vallone, Erica L R Butts, Carolyn R Hill, Margaret C Kline, Theresa Rinta, Amy M Gerow, Stacey R Allen, Christopher K Huber, John Teske, Benjamin Krenke, Martin Ensenberger, Patricia Fulmer, Cynthia Sprecher.
Abstract
As short tandem repeat markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex(®) 18D System was created for the direct amplification of buccal and blood samples from FTA(®) storage cards and reliably accommodates other sample materials. The PowerPlex(®) 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin along with four additional loci: Penta E, Penta D, D2S1338, and D19S433. To demonstrate suitability for human identification testing, the PowerPlex(®) 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWGDAM developmental validation guidelines. Given these results, PowerPlex(®) 18D System can confidently be used for forensic and human identification testing. Published by Elsevier Ireland Ltd.Entities:
Mesh：
Year: 2012 PMID： 22921483 DOI： 10.1016/j.fsigen.2012.07.008
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN： 1872-4973 Impact factor: 4.882