Literature DB >> 22921428

A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures.

Laura S Morris1, James Evans, Julian R Marchesi.   

Abstract

A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 22921428     DOI： 10.1016/j.mimet.2012.08.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Current functional metagenomic approaches only expand the existing protease sequence space, but does not presently add any novelty to it.

Authors:  Laura S Morris; Julian R Marchesi
Journal:  Curr Microbiol       Date:  2014-08-21       Impact factor: 2.188

2.  Comparative Transcriptomic Analysis of Streptococcus thermophilus TH1436 and TH1477 Showing Different Capability in the Use of Galactose.

Authors:  Sabrina Giaretta; Laura Treu; Veronica Vendramin; Vinícius da Silva Duarte; Armin Tarrah; Stefano Campanaro; Viviana Corich; Alessio Giacomini
Journal:  Front Microbiol       Date:  2018-08-07       Impact factor: 5.640

3.  Two-step functional screen on multiple proteinaceous substrates reveals temperature-robust proteases with a broad-substrate range.

Authors:  Antonio García-Moyano; Yuleima Diaz; José Navarro; David Almendral; Pål Puntervoll; Manuel Ferrer; Gro Elin Kjæreng Bjerga
Journal:  Appl Microbiol Biotechnol       Date:  2021-03-26       Impact factor: 4.813

4.  Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina.

Authors:  Stefany Castaldi; Claudia Petrillo; Giuliana Donadio; Fabrizio Dal Piaz; Alessio Cimmino; Marco Masi; Antonio Evidente; Rachele Isticato
Journal:  Int J Mol Sci       Date:  2021-03-24       Impact factor: 5.923
  4 in total

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