| Literature DB >> 22921316 |
Ghada Khawaja1, Thierry Buronfosse, Catherine Jamard, Fabien Abdul, Sylviane Guerret, Fabien Zoulim, Alain Luxembourg, Drew Hannaman, Claire F Evans, Daniel Hartmann, Lucyna Cova.
Abstract
This preclinical study investigated the therapeutic efficacy of electroporation (EP)-based delivery of plasmid DNA (pDNA) encoding viral proteins (envelope, core) and IFN-γ in the duck model of chronic hepatitis B virus (DHBV) infection. Importantly, only DNA EP-therapy resulted in a significant decrease in mean viremia titers and in intrahepatic covalently closed circular DNA (cccDNA) levels in chronic DHBV-carrier animals, compared with standard needle pDNA injection (SI). In addition, DNA EP-therapy stimulated in all virus-carriers a humoral response to DHBV preS protein, recognizing a broader range of major antigenic regions, including neutralizing epitopes, compared with SI. DNA EP-therapy led also to significant higher intrahepatic IFN-γ RNA levels in DHBV-carriers compared to other groups, in the absence of adverse effects. We provide the first evidence on DNA EP-therapy benefit in terms of hepadnaviral infection clearance and break of immune tolerance in virus-carriers, supporting its clinical application for chronic hepatitis B.Entities:
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Year: 2012 PMID： 22921316 DOI： 10.1016/j.virol.2012.07.014
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616