Literature DB >> 22921058

Evolution in microfluidic droplet.

Itay Levin, Amir Aharoni.   

Abstract

High-throughput screening (HTS) of enzymatic activity is important for directed evolution-based enzyme engineering. However, substrate and product diffusion can severely compromise these HTS assays. In this issue of Chemistry & Biology, Kintses and coworkers describe a microfluidic platform for the directed evolution of enzymes in droplets that allows for the screening of 10(7) mutants per round of evolution.
Copyright © 2012 Elsevier Ltd. All rights reserved.

Entities:  

Year:  2012        PMID： 22921058     DOI： 10.1016/j.chembiol.2012.08.004

Source DB:  PubMed          Journal:  Chem Biol        ISSN： 1074-5521


  3 in total

Review 1.  High-throughput droplet-based microfluidics for directed evolution of enzymes.

Authors:  Flora W Y Chiu; Stavros Stavrakis
Journal:  Electrophoresis       Date:  2019-08-29       Impact factor: 3.535

2.  One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution.

Authors:  Anastasia Zinchenko; Sean R A Devenish; Balint Kintses; Pierre-Yves Colin; Martin Fischlechner; Florian Hollfelder
Journal:  Anal Chem       Date:  2014-02-11       Impact factor: 6.986

Review 3.  Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

Authors:  Andrew Currin; Neil Swainston; Philip J Day; Douglas B Kell
Journal:  Chem Soc Rev       Date:  2015-03-07       Impact factor: 54.564
  3 in total

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