Literature DB >> 22914859

A novel microfluidic microplate as the next generation assay platform for enzyme linked immunoassays (ELISA).

Junhai Kai1, Aniruddha Puntambekar, Nelson Santiago, Se Hwan Lee, David W Sehy, Victor Moore, Jungyoup Han, Chong H Ahn.   

Abstract

In this work we introduce a novel microfluidic enzyme linked immunoassays (ELISA) microplate as the next generation assay platform for unparalleled assay performances. A combination of microfluidic technology with standard SBS-configured 96-well microplate architecture, in the form of microfluidic microplate technology, allows for the improvement of ELISA workflows, conservation of samples and reagents, improved reaction kinetics, and the ability to improve the sensitivity of the assay by multiple analyte loading. This paper presents the design and characterization of the microfluidic microplate, and its application in ELISA.

Mesh：

Year:  2012        PMID： 22914859     DOI： 10.1039/c2lc40585g

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  18 in total

1.  Smartphone instrument for portable enzyme-linked immunosorbent assays.

Authors:  Kenneth D Long; Hojeong Yu; Brian T Cunningham
Journal:  Biomed Opt Express       Date:  2014-10-01       Impact factor: 3.732

Review 2.  Next generation ligand binding assays-review of emerging technologies' capabilities to enhance throughput and multiplexing.

Authors:  Johanna Mora; Allison Given Chunyk; Mark Dysinger; Shobha Purushothama; Claude Ricks; Karolina Osterlund; Valerie Theobald
Journal:  AAPS J       Date:  2014-09-06       Impact factor: 4.009

3.  Digital microfluidic assay for protein detection.

Authors:  Janine Mok; Michael N Mindrinos; Ronald W Davis; Mehdi Javanmard
Journal:  Proc Natl Acad Sci U S A       Date:  2014-01-21       Impact factor: 11.205

Review 4.  Passive micropumping in microfluidics for point-of-care testing.

Authors:  Linfeng Xu; Anyang Wang; Xiangpeng Li; Kwang W Oh
Journal:  Biomicrofluidics       Date:  2020-05-27       Impact factor: 2.800

5.  Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay.

Authors:  Arnold Chen; Royal Wang; Candace R S Bever; Siyuan Xing; Bruce D Hammock; Tingrui Pan
Journal:  Biomicrofluidics       Date:  2014-11-05       Impact factor: 2.800

6.  Preprogrammed capillarity to passively control system-level sequential and parallel microfluidic flows.

Authors:  Sung-Jin Kim; Sophie Paczesny; Shuichi Takayama; Katsuo Kurabayashi
Journal:  Lab Chip       Date:  2013-06-07       Impact factor: 6.799

Review 7.  Microfluidics as a new tool in radiation biology.

Authors:  Jerome Lacombe; Shanna Leslie Phillips; Frederic Zenhausern
Journal:  Cancer Lett       Date:  2015-12-15       Impact factor: 8.679

Review 8.  Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics.

Authors:  Chandra K Dixit; Karteek Kadimisetty; Brunah A Otieno; Chi Tang; Spundana Malla; Colleen E Krause; James F Rusling
Journal:  Analyst       Date:  2015-11-03       Impact factor: 4.616

9.  Development of an automated on-chip bead-based ELISA platform.

Authors:  Jennifer Campbell; Nira Pollock; Andre Sharon; Alexis F Sauer-Budge
Journal:  Anal Methods       Date:  2015-08-13       Impact factor: 2.896

Review 10.  Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms.

Authors:  Maowei Dou; Sharma Timilsina Sanjay; Merwan Benhabib; Feng Xu; XiuJun Li
Journal:  Talanta       Date:  2015-05-06       Impact factor: 6.057
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