Literature DB >> 2291470

Skeletal keratan sulfate chain molecular weight calibration by high-performance gel-permeation chromatography.

J M Dickenson1, H G Morris, I A Nieduszynski, T N Huckerby.   

Abstract

A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gel-permeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 M NaCl and at 30 degrees C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2291470     DOI： 10.1016/0003-2697(90)90192-c

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  N.m.r. spectroscopic studies of fucose-containing oligosaccharides derived from keratanase digestion of articular cartilage keratan sulphates. Influence of fucose residues on keratanase cleavage.

Authors:  G H Tai; T N Huckerby; I A Nieduszynski
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

2.  A sub-population of keratan sulphates derived from bovine articular cartilage is capped with alpha(2-6)-linked N-acetylneuraminic acid residues. Affinity chromatography using immobilized Sambucus nigra lectin and characterization using 1H n.m.r. spectroscopy.

Authors:  G H Tai; H G Morris; G M Brown; T N Huckerby; I A Nieduszynski
Journal:  Biochem J       Date:  1992-08-15       Impact factor: 3.857

3.  Fucose content of keratan sulphates from bovine articular cartilage.

Authors:  G H Tai; G M Brown; H G Morris; T N Huckerby; I A Nieduszynski
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.