[Analysis of polymorphism on ribosomal internal transcribed spacer 2 in four species of sandflies with PCR-RFLP].

Internal transcribed spacer 2 region in 4 species of sandflies from China (Phlebotomus chinensis, Ph. wui, Ph. longiductus and Ph. alexandri) was genotyped by PCR-RFLP. Four kinds of RFLP band patterns were created by Dra I, that were A 450 bp, B 154 bp/127 bp/93 bp/66 bp/10 hp, C 311 bp/80 bp/60 bp, and D 353 bp/300 bp/160 bp/58 bp. There were identical pattern types in various sandflies, AA in Ph. chinensis, BB in Ph. alexandri, CC in Ph. wui, and DD in Ph. longiductus. The results indicated that PCR-RFLP can be used to identify the four species of Phlebotomus in China.