Literature DB >> 22907367

Affinity determination of biotinylated antibodies by flow cytometry.

Klervi Even-Desrumeaux1, Patrick Chames.   

Abstract

Affinity determination is a crucial step of an antibody characterization. Here, we describe a method for antibody affinity determination by flow cytometry, relying on the unique affinity of biotin for streptavidin for easy and efficient antibody labeling. Several labeling approaches are described and discussed in this chapter, including chemical and enzymatic (in vivo and in vitro) biotinylation. Finally, a procedure for K (D) determination by flow cytometry is precisely described.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22907367     DOI： 10.1007/978-1-61779-974-7_25

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Vaccine-Derived Neutralizing Antibodies to the Human Cytomegalovirus gH/gL Pentamer Potently Block Primary Cytotrophoblast Infection.

Authors:  Flavia Chiuppesi; Felix Wussow; Erica Johnson; Chao Bian; Meng Zhuo; Augustine Rajakumar; Peter A Barry; William J Britt; Rana Chakraborty; Don J Diamond
Journal:  J Virol       Date:  2015-09-16       Impact factor: 5.103

2.  Thyroid Disrupting Chemicals in Mixture Perturb Thymocyte Differentiation in Xenopus laevis Tadpoles.

Authors:  Connor C McGuire; B Paige Lawrence; Jacques Robert
Journal:  Toxicol Sci       Date:  2021-05-27       Impact factor: 4.849

3.  A FcγRIII-engaging bispecific antibody expands the range of HER2-expressing breast tumors eligible to antibody therapy.

Authors:  Marc Turini; Patrick Chames; Pierre Bruhns; Daniel Baty; Brigitte Kerfelec
Journal:  Oncotarget       Date:  2014-07-30
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.