| Literature DB >> 22907356 |
Sang Jick Kim1, Hyo Jeong Hong.
Abstract
Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world's first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody.Entities:
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Year: 2012 PMID： 22907356 DOI： 10.1007/978-1-61779-974-7_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745