| Literature DB >> 22907352 |
Romain Rouet1, Kip Dudgeon, Daniel Christ.
Abstract
Human antibody single domains are a promising new class of antibody fragments. Here we describe methods for the cloning of human V(H) and V(L) genes into phage and phagemid vectors. Furthermore, we provide detailed protocols for the generation of single domain antibody libraries by Kunkel mutagenesis and the analysis of diversity by DNA sequencing and superantigen binding.Entities:
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Year: 2012 PMID： 22907352 DOI： 10.1007/978-1-61779-974-7_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745