Literature DB >> 22902388

Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production.

Maria Elisa Rodrigues1, Ana Rita Costa, Mariana Henriques, Joana Azeredo, Rosário Oliveira.   

Abstract

The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 22902388     DOI： 10.1016/j.ijpharm.2012.08.002

Source DB:  PubMed          Journal:  Int J Pharm        ISSN： 0378-5173            Impact factor:   5.875


  8 in total

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Authors:  Sandra Fernanda Suárez-Patiño; Thaissa Consoni Bernardino; Eutimio Gustavo Fernández Núñez; Renato Mancini Astray; Carlos Augusto Pereira; Hugo R Soares; Ana S Coroadinha; Soraia Attie Calil Jorge
Journal:  Cytotechnology       Date:  2019-08-17       Impact factor: 2.058

2.  Modular development enables rapid design of media for alternative hosts.

Authors:  Andrew M Biedermann; Isabella R Gengaro; Sergio A Rodriguez-Aponte; Kerry R Love; J Christopher Love
Journal:  Biotechnol Bioeng       Date:  2021-10-18       Impact factor: 4.395

3.  Using simple models to describe the kinetics of growth, glucose consumption, and monoclonal antibody formation in naive and infliximab producer CHO cells.

Authors:  Julián López-Meza; Diana Araíz-Hernández; Leydi Maribel Carrillo-Cocom; Felipe López-Pacheco; María Del Refugio Rocha-Pizaña; Mario Moisés Alvarez
Journal:  Cytotechnology       Date:  2015-06-20       Impact factor: 2.058

4.  Selection of chemically defined media for CHO cell fed-batch culture processes.

Authors:  Xiao Pan; Mathieu Streefland; Ciska Dalm; René H Wijffels; Dirk E Martens
Journal:  Cytotechnology       Date:  2016-11-29       Impact factor: 2.058

5.  Dynamic Modeling of CHO Cell Metabolism Using the Hybrid Cybernetic Approach With a Novel Elementary Mode Analysis Strategy.

Authors:  Juan A Martínez; Dubhe B Bulté; Martha A Contreras; Laura A Palomares; Octavio T Ramírez
Journal:  Front Bioeng Biotechnol       Date:  2020-04-15

Review 6.  Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells.

Authors:  Weifeng Li; Zhenlin Fan; Yan Lin; Tian-Yun Wang
Journal:  Front Bioeng Biotechnol       Date:  2021-03-15

7.  The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles.

Authors:  Mi Jang; Ellen Sofie Pete; Per Bruheim
Journal:  Front Bioeng Biotechnol       Date:  2022-09-06

8.  Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of "Difficult-to-Express" Proteins and Future Perspectives.

Authors:  Lena Thoring; Doreen A Wüstenhagen; Maria Borowiak; Marlitt Stech; Andrei Sonnabend; Stefan Kubick
Journal:  PLoS One       Date:  2016-09-29       Impact factor: 3.240
  8 in total

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