AIM: The laboratory diagnosis of pulmonary tuberculosis (TB) and tuberculous meningitis (TBM) is particularly challenging. The aim of the present work is to develop an immunoassay for the diagnosis of TB infection, using synthetic peptides of antigen (Ag) 85 complex of M. tuberculosis (Mtb) H37Rv. METHODS: Four peptides (7-10 amino acids long) corresponding to group-specific epitopes of Ag 85 complex of Mtb were synthesized. All peptides were evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoreactivity with sera and CSF samples of TB and TBM patients respectively. The diagnostic value of the four peptides was evaluated in both the samples. RESULTS: It was observed that Ag 85 peptide 1, 3 and 4 had the highest positive rates in the pulmonary patients; however, Ag 85 peptide 1 and 2 had shown good positivity in the TBM subjects. CONCLUSIONS: The synthetic peptide based ELISA using Ag 85 complex peptides is a sensitive, specific, rapid and cost effective immunoassay for early diagnosis of pulmonary and extrapulmonary TB. In addition, these synthetic peptides are comparatively easy to produce in a reproducible manner compared with the whole antigen.
AIM: The laboratory diagnosis of pulmonary tuberculosis (TB) and tuberculous meningitis (TBM) is particularly challenging. The aim of the present work is to develop an immunoassay for the diagnosis of TB infection, using synthetic peptides of antigen (Ag) 85 complex of M. tuberculosis (Mtb) H37Rv. METHODS: Four peptides (7-10 amino acids long) corresponding to group-specific epitopes of Ag 85 complex of Mtb were synthesized. All peptides were evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoreactivity with sera and CSF samples of TB and TBM patients respectively. The diagnostic value of the four peptides was evaluated in both the samples. RESULTS: It was observed that Ag 85 peptide 1, 3 and 4 had the highest positive rates in the pulmonarypatients; however, Ag 85 peptide 1 and 2 had shown good positivity in the TBM subjects. CONCLUSIONS: The synthetic peptide based ELISA using Ag 85 complex peptides is a sensitive, specific, rapid and cost effective immunoassay for early diagnosis of pulmonary and extrapulmonary TB. In addition, these synthetic peptides are comparatively easy to produce in a reproducible manner compared with the whole antigen.
Authors: Ana Paula Junqueira-Kipnis; Fábio Muniz de Oliveira; Monalisa Martins Trentini; Sangeeta Tiwari; Bing Chen; Danilo Pires Resende; Bruna D S Silva; Mei Chen; Lydia Tesfa; William R Jacobs; André Kipnis Journal: PLoS One Date: 2013-11-08 Impact factor: 3.240
Authors: Aliabbas A Husain; Shubhangi R Warke; Dewanand R Kalorey; Hatim F Daginawala; Girdhar M Taori; Rajpal S Kashyap Journal: Clin Exp Vaccine Res Date: 2015-01-30
Authors: Adeliane Castro da Costa; Abadio de Oliveira Costa-Júnior; Fábio Muniz de Oliveira; Sarah Veloso Nogueira; Joseane Damaceno Rosa; Danilo Pires Resende; André Kipnis; Ana Paula Junqueira-Kipnis Journal: PLoS One Date: 2014-11-14 Impact factor: 3.240
Authors: Wei Zhang; Qingbo Shu; Zhen Zhao; Jia Fan; Christopher J Lyon; Adrian M Zelazny; Ye Hu Journal: Clin Proteomics Date: 2018-01-08 Impact factor: 3.988